The subject of the studies were F1 and F2 hybrids originating from reciprocal crosses of the highlysine mutant C-67-7 and cultivar Miranda, the forms with different hordein polypeptide spectra. The method of SDS gel electrophoresis was used for an analysis of the main hordein fractions, hordein-1 and hordein-2, from single kernels of barley. It was found that polypeptide spectra of hordein-1 and hordein-2 of the high lysine mutant C-68-7 are determined by codominant alleles at two loci, which are moderately linked.
Data on segregation of the lys 3 b gene in the offsprings of crosses between the mutant Ris? 18 and the barley forms with plump kernels and high hordein content are presented.Hordein phenotype, and indirectly lysine, were determined by visual screening method.In the analysed materials all plump kernels were high-hordein (low lisine), and all shrunken kernels were low hordein (high-lysine).Genetic analysis showed a 3:1 Mendelian phenotypic ratio of normal and mutant F2 kernels in most of the analysed crosses.
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