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Effect of genotype and media composition on embryoid induction and plant regeneration from anther culture in triticale

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Slusarkiewicz J. A., Ponitka A.
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vol. 38
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issue 3
253-258
EN
Anthers of twenty triticale genotypes were cultured on three different media: 1 - PII (CHUANG et al. 1978) with increased 2,4 D to 2 mg L 1 and agarose 6 g L 1; 2 - Macro , micronutrients and vitamines like in MN6 (CHU, HILL 1988) + 2 mg L 1 2.4 D + 0.5 mg L 1 KIN + 5 mg L 1 FeEDTA + 90 g L 1 sucrose; 3 - Macro , micronutrients and vitamines like in MN6 (CHU, HILL, 1988) + 2 mg L 1 2.4 D + 0.5 mg L 1 KIN + 5 mg L 1 FeEDTA + 120 g L 1 sucrose. Embryoid induction and plant regeneration were influenced by donor plant genotype and induction medium. Medium 1 was the best for embryoid induction, while for green plant regeneration the best were media 1 and 2. Out of 300 anthers from each genotype plated on each of the three media, 64 1250, 12 486 and 6 212 somatic embryos and 8 86, 3 136 and 1 26 green plants were recorded, on media 1, 2 and 3, respectively.
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