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1

Strucutral studies on carbohydrate part of Hafnia alvei lipopolysaccharides

100%
Katzenellenbogen E.
Postępy Higieny i Medycyny Doświadczalnej
|
1996
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vol. 50
|
issue 5
419-429
EN
The structures of 14 O-specific polysaccharides isolated from Hafnia alvei lipopolysaccharides have been described. The structures of the common core existing in most Hafnia alvei strains examined so far and the core-like trisaccharide found in some Hafnia alvei strains have been also presented.
2

Structural and serological charaqcterization of Hafnia alvei PCM 1185 and 1199 O-specific polysaccharides

100%
K?bler J., Mieszala M., Katzenellenbogen E., Gamian A., Romanowska E.
Postępy Higieny i Medycyny Doświadczalnej
|
1996
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vol. 50
|
issue 5
515-517
EN
This work describes results of studies of O-specific oligosaccharide repeating units from lipopolysaccharides of two related Hafnia alvei serotypes. The linkage between O-antigen and the core region in PCM 1199 LPS has been also established. The O-acetyl residues present in the polysaccharides are involved in formation of epitopes.
3

Immunochemical studies of the lipopolysaccharides of Hafnia alvei PCM 1219 and other strains with the O-antigens containing D-glucose 1-phosphate and 2-deoxy-2-[(R)-3-hydroxybutyramido]-D-glucose

88%
Katzenellenbogen E., Kocharova N. A., Korzeniowska-Kowal A., Gamian A., Bogulska M., Szostko B., Shashkov A. S., Knirel Y. A.
Archivum Immunologiae et Therapiae Experimentalis
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2008
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vol. 56
|
issue 5
347-352
EN
Introduction: Hafnia alvei is the only species of the genus Hafnia, which belongs to the family of Enterobacteriaceae. These Gram-negative bacteria are commonly distributed in the natural environment and are often the cause of human opportunistic infections. Their lipopolysaccharides (LPSs) are important surface antigens which are responsible for the serological specificity and numerous cross-reactions with other enterobacterial genera. So far, 29 different O-polysaccharide (OPS, O-antigen) structures in Hafnia LPSs have been established and for some of them the molecular basis of the serological activity has been elucidated. Materials and Methods: OPS from H. alvei strain PCM 1219 was obtained by mild acid hydrolysis of the LPS followed by gel permeation chromatography of carbohydrate material on Sephadex G-50 column. The polysaccharide structure was determined using chemical methods as well as 13C NMR and 1H NMR spectroscopy. For serological studies, SDS-PAGE, immunoblotting, and passive hemagglutination tests were used. Results: The serological studies revealed a cross-reactivity of the LPSs of H. alvei PCM 1219 and a group of H. alvei strains with an O-antigen containing D-glucose 1-phosphate and [(R)-3-hydroxybutyramido]-D-glucose. The following structure of the OPS was established: 2)-alpha-d-Glcp-(1-PO4-6)-alpha-d-GlcpNAcyl-(14)-alpha-d-GalpNAc-(13)-beta-d-GalpNAc-(1- > 3---- OAc6<-1 alpga-d-Glcp where Acyl stands for (R)-3-hydroxybutyryl and the degree of O-acetylation is 70%. The structure of the core oligosaccharide was found to be typical of the genus Hafnia. Conclusions: Based on the OPS structure and serological results it was concluded that H. alvei strain PCM 1219 should be classified in the same serogroup as the H. alvei type strain ATCC 13337 and five other strains containing D-glucose 1-phosphate and 2-deoxy-2-[(R)-3-hydroxybutyramido]-D-glucose in their O-antigens.
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