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Expression of avian influenza haemagglutinin (H5) and chicken interleukin 2 (chIL-2) under control of the ptcB promoter in Lactococcus lactis

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Szatraj K., Szczepankowska A., Sączyńska V., Florys K., Gromadzka B., Łepek K., Płucienniczak G., Szewczyk B., Zagórski-Ostoja W., Bardowski J.

2014

vol. 61

issue 3

609-614

Gram-positive and nonpathogenic lactic acid bacteria (LAB) are considered to be promising candidates for the development of new, safe systems of heterologous protein expression. Recombinant LAB has been shown to induce specific local and systemic immune response against selected pathogens, and could be a good alternative to classical attenuated carriers. The main goal of our study was to express the avian influenza haemagglutinin (H5) and chicken interleukin 2 (chIL-2) in Lactococcus lactis. Results of this study were anticipated to lead to construction of lactococcal strain(s) with potential vaccine properties against the avian influenza A (H5N1) virus. Expression of the cloned H5 gene, its His-tagged variant and chIL-2 gene, under the control of the ptcB gene promoter was attested by RT-PCR on transcriptional level and Western or dot blot analysis on translational level, demonstrating that system can be an attractive solution for production of heterologous proteins. The results of the preliminary animal trial conducted in mice are a promising step toward development of a vaccine against avian bird flu using Lactococcus lactis cells as antigen carriers.

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1 Acta Biochimica Polonica

1 2014

1 Bardowski J.

1 Florys K.

1 Gromadzka B.

1 Płucienniczak G.

1 Szatraj K.

1 Szczepankowska A.

1 Szewczyk B.

1 Sączyńska V.

1 Zagórski-Ostoja W.

1 Łepek K.

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Expression of avian influenza haemagglutinin (H5) and chicken interleukin 2 (chIL-2) under control of the ptcB promoter in Lactococcus lactis