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Qualitative and quantitative analysis of gallic acid in Alchemilla vulgaris, Allium ursinum, Acorus calamus and Solidago virga-aurea by chip-electrospray ionization mass spectrometry and high performance liquid chromatography

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Condrat D., Mosoarca C., Zamfir A., Crişan F., Szabo M., Lupea A.
Open Chemistry
|
2010
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vol. 8
|
issue 3
530-535
EN
This study presents the results obtained from qualitative and quantitative analysis of gallic acid from hydro-alcoholic extracts (methanol, ethanol) of plants from Plantae regnum. Plant qualitative analysis was performed using a novel mass spectrometric (MS) method based on fully automated chip-nanoelectrospray ionization (nanoESI) high capacity ion trap (HCT) while quantitative analysis was carried out by high performance liquid chromatography (HPLC). These methods were applied to Alchemilla vulgaris - common lady’s-mantle (aerial part), Allium ursinum - bear’s garlic (leaves), Acorus calamus - common sweet flag (roots), Solidago virga-aurea - goldenrod (aerial part). Obtained results indicated that methanol extracts (96%, 80%) have a gallic acid content ranging between 0.0011–0.0576 mg mL−1 extract while the ethanol extracts (96%, 60%) exhibit a gallic acid concentration that varies between 0.0010–0.0182 mg mL−1 extract. [...]
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A comparative study on the antioxidant efficiency of nine compounds commonly used as standards in antioxidant assays of extracts from medicinal plants and functional foods

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Quessaugy K., Cumbane P.
World News of Natural Sciences
|
2023
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vol. 46
30-45
EN
The detection of antioxidant activity in plant extracts or in pure compounds can be performed by a large number of methods with different reaction mechanisms, however, the criteria for choosing comparative standards are still not consensual. Thus, the present work intends to compare the antioxidant efficiency of nine substances, namely, gallic acid (GA), pyrogallol (PyG), propyl gallate (nPG), tannic acid (TA), quercetin (Qtn), rutin (Rut), ascorbic acid (Asc), Trolox (6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid) and butyl hydroxytoluene (BHT) using methods, (1) Ferric Reducing Antioxidant Power (FRAP) Assay, (2) Ferric Reducing Power (FRP), (3) Ferric-Ferrozine Antioxidant Capacity (FFAC), (4) Total Phosphomolybdenum Antioxidant Capacity (TAC) and (5) Radical Cation Elimination Assay 2,2' Azinobis(3 Ethylbenzothiazoline 6 Sulfonic Acid) (ABTS). Antioxidant efficacy by the 1,1-diphenyl-2-picrylhydrazine free radical scavenging method was previously described in a preliminary study. The results show that the maximum effectiveness was exhibited by PyG in the ABTS (0.425 ± 0.005 µM) and TAC (0.872 ± 0.075 µM) methods, Qtn in the FRP (5.776 ± 0.020 µM) and FFAC (20.390 ± 0.291 µM) methods and GA in the FRAP (6.765 ± 0.086 µM) and DPPH (1.105 ± 0.003 µM) methods. The results found in this study reveal that the effectiveness of a standard depends on the method applied, and the antioxidant activity of the same standard may present differences between the methods, which suggests that the selection of a comparative standard for the antioxidant activity tests of the extracts of plants or functional foods must be made according to the method to be applied.
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