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1

Conditions for efficient induction and maintenance of callus cultures Vaccinium corymbosum var. Bluecrop and its accumulation of polyphenolic compounds

100%

Dembinska-Migas W., Luczkiewicz M., Migas P., Cisowski W.

Biotechnologia

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1998

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issue 2

174-185

EN

Zimmerman & Bromme and Schenk & Hildebrandt media with unchanged content of plant growth regulators in the basic medium, were used to develop the optimum conditions for efficient production of callus tissue of Vaccinium corymbosum var. Bluecrop. It has been determined that, of the auxins used, it was 2,4-D which has the most beneficial effect on callus tissue growth. Zeatin was found to regulate the water content of the tissue obtained. Quercetin-3-O-glucoside, as well as quercetin-3-O-galactoside were identified and analysed quantitatively in the callus tissue from in vitro cultures. Moreover, the following phenolic acids were identified in the callus with the use of chromatography: ferulic acid, p-hydroxybenzoic acid, p-coumaric acid, as well as gallic acid and protocatechuic acid which were also analysed quantitatively in the researched tissue material. Phytochemical monitoring of the propagated tissues for different production media (HPLC analysis) did not show any relationship between the plant growth regulators used and the analysed secondary metabolites.

2

Elevated TGF-beta1 concentration in bronchoalveolar lavage fluid from patients with primary lung cancer

100%

Domag?a-Kulawik J., Hoser G., Safianowska A., Grubek- J. H., Chazan R.

Archivum Immunologiae et Therapiae Experimentalis

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2006

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vol. 54

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issue 2

143-147

EN

Introduction: Transforming growth factor (TGF)-beta is one of numerous inhibitory factors produced by cancer cells that regulate antitumor immunity. The aim of this study was to evaluate TGF-beta1 levels and lymphocyte subsets in the broncholaveolar lavage fluid (BALF) of patients with primary lung cancer and to analyze the interdependence of these parameters. Materials and Methods: BALF samples were collected from 38 patients with primary lung cancer prior to treatment and from 23 healthy volunteers. Concentrations of TGF-beta1 were measured in two independent lots of samples using a commercially available sandwich ELISA kit after concentration of the supernatants. Differential cell counts in the BALF were performed on slides stained with the May Grnwald Giemsa method. Flow cytometry with monoclonal antibodies was applied for lymphocyte phenotyping. Results: A higher level of TGF-beta1 in the BALF of patients compared with the healthy subjects was observed in both lots of samples (3.232.96 pg/ml vs. 1.050.95 pg/ml, p<0.05, and 16.119.3 pg/ml vs. 10.111.1 pg/m,, respectively, difference not significant). There was significant positive correlation of the TGF-beta1 level with the proportion of lymphocytes and negative correlation with both the proportion of macrophages and the percentage of cytotoxic and activated T lymphocytes. Conclusions: Our findings confirmed that TGF-beta takes part in the local response in the course of primary lung cancer.

3

Significance of tumor cell ? receptors in human cancer

100%

Zeromski J.

Archivum Immunologiae et Therapiae Experimentalis

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2002

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vol. 50

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issue 2

105-110

EN

Every tumor cell is equipped with an array of biologically active surface molecules, and several these function as receptors for various ligands. They include MHC, or in the case of humans, HLA antigens, cytokine receptors, cell-adhesion molecules, growth factor receptors, Fas/Fas-ligand molecules and others. Their expressions are a subject to alterations, usually to the advantage of tumor growth and spread. Some appear on tumor cells de novo, having no counterparts on the respective normal cells. Detailed knowledge about the expression of tumor-cell receptors and their genotypes, in particular of cancerous ones, may provide information essential for the creation of tools for specific tumor immunotherapy.

4

Expressive gene structures of proper and modified genes

88%

Lipinski D., Szalata M., Kalak R., Plawski A., Nuc K., Kala M., Juzwa W., Slomska K., Gronek P., Jura J., Smorag Z., Pienkowski M., Slomski R.

Biotechnologia

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2003

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issue 1

48-73

EN

The genetic construct WAP 6xHisHGH containing the gene encoding human growth hormone (hGH) and WAP promoter expressed in mammary gland of animals was prepared. The 5? end of the gene was modified by the addition of sequence encoding six histidine residues and the sequence recognized by thrombin. In this way, the growth hormone can be easily purified by affinity chromatography and cleaved with thrombin to an active form. In the next step, the genetic construct was introduced by microinjection into male pronuclei of fertilized oocytes. Transgene was detected in male rabbit of F0 generation (number 61). Twelve offspring of founder rabbit of generation F1 indicated transgene sequences. The presence of growth hormone was revealed in the samples of milk accumulated during the lactation of females of F1 generation. The genetic constructs containing chain 1 and chain 2 of Feld1, and the major allergen produced by cat (Fedlis domesticus) were prepared. Both genes were inactivated by introduction into the sequences a positive selectable marker aminoglycoside phosphotransferase (resistant to neomycin). Outside the region of homology to Feld1 chain 1 and chain 2 genes, the negative selectable marker ? thymidine kinase gene was introduced. The genetic constructs pNTKFd1 and pNTKFd2 can be used in further experiments involving the inactivation of Feld1 genes in cat cells. Both genes were modified by site-directed mutagenesis using megastarter with Stop codon for premature termination of translation. The presence of mutation was confirmed by sequencing. The genetic constructs with human hGH gene and cat Feld1 gene were introduced into the bovine and cat fetal fibroblasts respectively in co-transfection with plasmid pGT-N29 containing positive selectable marker by lipofection, precipitation and electroinjection methods. After the selection, surviving cells were subjected to further molecular analysis. The stabile incorporation of the genetic constructs WAP 6xHisHGH and WAPHGH into the genome were observed.

5

Stimulation of anthocyanin production by two-phase growth system, in callus of Rudbeckia hirta

75%

Luczkiewicz M., Cisowski W.

Biotechnologia

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1999

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issue 4

199-210

EN

Biotechnological research to achieve and then increase anthocyanin production in callus tissue of R. hirta L., involved testing a number of growth media, modified with growth regulators. The evaluation of the growth of propagated tissues, their water contents and in particular their ability to produce anthocyanins, led to the development of an original two-phase growth system. This new system has the advantages of both growth-stimulating media and pigment production media. As a result of the research, callus tissue was obtained on a two-phase growth medium, which was made of modified Schenk-Hildebrandt medium (growth phase) and Miller's medium (production phase). The callus synthesised a 12-component anthocyanin compound complex, which constituted 2,18% of dry mass. This is a considerable amount compared to 0,28% in the natural plant. Phytochemical analysis (TLC, HPLC, PC, UV, 1H-NMR) of the anthocyanin complex isolated from callus produced with the two-phase system proved that the dominating compounds in the pigment complex were: cyanidin-3-0-(6-0-malonyl-b -D-glucopyranoside) and cyanidin-3-0-(b-D-glucopyranoside).

6

Heparin and atherosclerosis

75%

Stajszczak M., Gminski J.

Postępy Higieny i Medycyny Doświadczalnej

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1994

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vol. 48

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issue 3

275-290

EN

Heparin is a highly sulfated glycosaminoglycan with many functions such as antilipemic and antithrombotic.In spite os these activities heparin is able to inhibit vacsular smooth muscle cells proliferation and mmigration what seems to be very important event in the pathogenesis of atherosclerosos.The molecular mechanism of the action of heparin on smooth muscle cells is not yet understood.Heparin inhibits growth factors binding to their receptors,oncogenes expressionand has influence on the extracellular matrix protein deposition in the artery wall.

Refine search results

Conditions for efficient induction and maintenance of callus cultures Vaccinium corymbosum var. Bluecrop and its accumulation of polyphenolic compounds

Elevated TGF-beta1 concentration in bronchoalveolar lavage fluid from patients with primary lung cancer

Significance of tumor cell ? receptors in human cancer

Expressive gene structures of proper and modified genes

Stimulation of anthocyanin production by two-phase growth system, in callus of Rudbeckia hirta

Heparin and atherosclerosis