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1

RAPD-PCR analysis of various goose populations

100%
Maciuszonek A., Grajewski B., Bednarczyk M.
Folia Biologica
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2005
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vol. 53
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issue 1-2
83-85
EN
The aim of this study was to genetically analyse by the RAPD-PCR method four indigenous Polish goose breeds, Kartuska (Ka), Lubelska (Lu), Kielecka (Ki) and Podkarpacka (Pd), in order to determine the band-sharing frequency as well as bands characteristic of the evaluated breeds. The birds were maintained as conservative flocks, accounting for a reserve of genetic resources. A total of 102 scorable bands were obtained, their number ranging from 0 to 8, depending on one of seven primers used and the group of birds analyzed, within a mean of 3.64. For each genetic group specific bands with given primers were obtained, suggesting their potential for use as population-specific markers, especially in ex-situ conservation methods. The results also suggest that keeping endangered geese as separate flocks is relevant for their preservation.
2

Description of the Anser anser goose karyotype

100%
Wojcik E., Smalec E.
Folia Biologica
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2007
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vol. 55
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issue 1-2
35-40
EN
The karyotype of the Italian goose originating from Anser anser was characterised on the basis of R and C bands. Chromosomal preparations obtained from an in vitro culture of blood lymphocytes were stained with the RBG and CBG techniques. The RBG technique enabled the analysis of the structure of nine pairs of chromosomes whereas the CBG technique ? fourteen pairs of chromosomes from the total of eighty goose chromosomes. The morphology and the R and C banding patterns were described. The size and arrangement of the blocks of constitutive chromatin were determined. Ideograms of R and C banded patterns were drawn. The morphological structure of the analysed chromosomes was evaluated.
3

The structure of the synaptonemal complexes in meiocytes of European domestic goose (Anser anser)

100%
Andraszek K., Smalec E., Czyzewska D.
Folia Biologica
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2008
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vol. 56
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issue 3-4
139-147
EN
The synaptonemal complex (SC) is a protein structure which binds two homologues during rophase of the firstmeiotic division and assures the correct course of genetic recombination. The demonstration and identification of SCs in European domestic goose Anser anser was the aim of the current research. Standard techniques of SC identification do not allow for an analysis of their molecular structure. In meiotic cells of one-day-old nestlings and 17-week-old geese the haploid number of bivalents, darkly stained kinetochores and subtelomeric regions may be evidence for the presence of SCs. Experimental chromosome staining with the DAPI fluorochrome permitted the observation of the characteristic ladder-like structure of SCs and the course of synapsis formation within homologues from early leptotene to their degradation in late pachytene. The detailed molecular structure of synaptonemal complexes can be analysed with an electron or scanning microscope only. Because the bivalent is a direct result of the complex.s presence, in the literature the presence of bivalents is equivalent to the term.synaptonemal complex.. However, the bivalent and the SC are two different structures.
4
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Effects of pituitary adenylate cyclase-activating polypeptide (PACAP) on cyclic AMP formation in the duck and goose brain

86%
Nowak J. Z., Kuba K., Zawilska J. B.
Acta Neurobiologiae Experimentalis
|
2000
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vol. 60
|
issue 2
209-214
EN
Two molecular forms of pituitary adenylate cyclase-activating polypeptide (PACAP), i.e., PACAP27 and PACAP38 (0.0001-1 muM), as well as vasoactive intestinal polypeptide (VIP; 0.1-3 muM), have been studied for their effects on cyclic AMP formation in the hypothalamus and cerebral cortex of duck and goose. All three peptides concentration-dependently stimulated cyclic AMP production in the tested brain regions of 2-3-weeks-old (young) ducks, with VIP showing at least one order of magnitude weaker activity than PACAP. This characteristics suggests the existence in the duck's brain of adenylyl cyclase-linked PAC1 receptors. Both forms of PACAP also stimulated the nucleotide formation in the cerebral cortex and hypothalamus of 5-6-months-old (adult) ducks or geese grown under natural environment. The peptides-evoked effects in adult and young ducks were comparable, and clearly greater than those found in adult geese. The present data extend our recent observations made on chicks, and suggest PACAP to be a potent stimulator of the cyclic AMP generation in the avian central nervous system.
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