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EN
In the last ten years, the global area of commercially grown, genetically modified (GM) crops has increased more than 50-times to over 90 mln hectares. GM plants are cultivated in twenty one countries (six European). Despite this high adoption rate and future promises, there is still a multitude of concerns about the impact of GM-crops on natural and agricultural environment. In this article, we present: a) current definition of crop co-existence, b) results of the studies on risk assessment and safe co-existence rules, as well as c) examples of wildernesses of public opinion.
EN
Intensified production and release of GMOs into the environment and market are correlated with the development of evaluation requirement and tools for GMO screening. In the last years, application of integrated approaches based on molecular detection techniques and bioinformatics tools has been noted. The examples of bioinformatics system, methods, and IT achievements are described in this paper.
EN
There is a constant expectation for fast improvement of livestock production and human health care products. The advent of DNA recombinant technology and the possibility of gene transfer between organisms of distinct species, or even distinct phylogenic kingdoms, has opened a wide range of possibilities. Nowadays we can produce human insulin in bacteria or human coagulation factors in cattle milk. The recent advances in gene transfer, animal cloning, and assisted reproductive techniques have partly fulfilled the expectation in the field of livestock transgenesis. This paper reviews the recent advances and applications of transgenesis in livestock and their derivative products. At first, the state of art and the techniques that enhance the efficiency of livestock transgenesis are presented. The consequent reduction in the cost and time necessary to reach a final product has enabled the multiplication of transgenic prototypes around the world. We also analyze here some emerging applications of livestock transgenesis in the field of pharmacology, meat and dairy industry, xenotransplantation, and human disease modeling. Finally, some bioethical and commercial concerns raised by the transgenesis applications are discussed.
EN
Phytoremediation is a field of science and technology that uses plants to clean up polluted soil and water. Metal hyperaccumulator plants are naturally capable of accumulating trace elements, primarily Ni, Zn, Cd, As or Se in their above-ground tissues, without developing any toxicity symptoms. Although these plants appear to have ideal properties for phytoextraction, most of them produce little biomass and grow only in ecosystems that are characteristic for them. The introduction of novel traits into high biomass plants using a transgenic approach is a promising strategy for the development of effective phytoremediation technologies. A number of transgenic plants have been generated in an attempt to modify: (a) trace element uptake from environment, (b) transport to organelles, (c) allocation within the plants, (d) synthesis of metal complexing ligands present in the cell or exported to the apoplast and/or environment, (e) metabolism of the metal containing a compound. Many experimental results demonstrate that a single-gene plant transformation rarely leads to intended phenotypes. In this paper, we present some selected results dealing with suitability of genetically modified plants for phytoremediation.
5
80%
|
2016
|
vol. 63
|
issue 4
731-735
EN
Conversion of scientific achievements to market a product is a key issue and the best description of significance of science for society. In the case of experts in the natural sciences in Poland, we observe a high intellectual potential of researchers and several scientific discoveries. However, Polish inventions are very rarely available on the market and the number of national and international patent applications done by Polish scientists is very limited. For the development of bioeconomy, the progress in biotechnology is critical.
EN
There is a constant expectation for fast improvement of livestock production and human health care products. The advent of DNA recombinant technology and the possibility of gene transfer between organisms of distinct species, or even distinct phylogenic kingdoms, has opened a wide range of possibilities. Nowadays we can produce human insulin in bacteria or human coagulation factors in cattle milk. The recent advances in gene transfer, animal cloning, and assisted reproductive techniques have partly fulfilled the expectation in the field of livestock transgenesis. This paper reviews the recent advances and applications of transgenesis in livestock and their derivative products. At first, the state of art and the techniques that enhance the efficiency of livestock transgenesis are presented. The consequent reduction in the cost and time necessary to reach a final product has enabled the multiplication of transgenic prototypes around the world. We also analyze here some emerging applications of livestock transgenesis in the field of pharmacology, meat and dairy industry, xenotransplantation, and human disease modeling. Finally, some bioethical and commercial concerns raised by the transgenesis applications are discussed.
EN
In 2007 Polish farmers produced 320 ha of genetically modified corn. About 85% of Polish farmers want to have freedom of choice and in 2007 over 70% are ready to breed GM corn. It is important to know, that Polish farmers correctly recognize the profits from GM plants. About 90% of farmers expect solid information from the state administration and labeling of GM products.
EN
Leishmaniasis causes significant morbidity and mortality worldwide, constituting an important public health problem. Leishmania infections cause a wide spectrum of diseases, ranging in severity from spontaneously healing skin lesions to fatal visceral disease. Attempts to develop an effective vaccine to control leishmaniasis have been shown to be feasible, but no vaccine is in active clinical use. The ability to create genetically modified parasites by eliminating virulence or essential genes is considered a powerful alternative in the development of an effective protective vaccine. Here, recent findings related to genetically defined live attenuated Leishmania parasites as promising vaccine candidates are reviewed.
EN
Rapeseed (Brassica napus L.) is the most important oilseed plant in Poland. Genetically modified (GM) rapeseed is planted on 18% of total rapeseed area in the world. The most frequent genetic modifications in rapeseed gave transgenic lines that are herbicide tolerant or have altered fatty acid composition. Coexistence of conventional and GM rapeseed is difficult due to several facts related to biology of this species. Contamination of rapeseed conventional varieties with rapeseed GM seems to be unavoidable during flowering time, harvest, storage and transport. For this reason monitoring of GM rapeseed in Poland is very important. At the moment no validated method is available for quantification of GM in rapeseed. Therefore development and validation of qualitative methods for rapeseed transgenic lines in routine food and feed analysis are necessary.
EN
Several DNA and protein-based analytical methods for GMOs detection have been established so far. For the detection of GMOs at the DNA level, mostly PCR-based methods are used, whereas at protein level ELISA and lateral strips are predominant. The choice of target sequence motif is the most important factor controlling the specificity of the PCR method. This review summarizes most widely used GMO analysis technologies and point out new areas of analytical investigations.
EN
European Community regulations concerning the control of use and distribution of genetically modified organisms and products resulted in creation of European and National GMO Laboratories Networks, which are specialized in GMO identification and quantification in food, feed and seed. The analyses are mainly based on PCR methods, however, cheaper and less time consuming methods are elaborated for GMO detection. This article summarizes the research on electrochemical biosensors based on nucleic acids hybridization and the perspectives these tools create.
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