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1

Evaluation of Thermus ruber as a source of thermostable (-glucosidase useful for production of glucose syrups

100%
Sinkiewicz I., Synowiecki J.
Biotechnologia
|
2009
|
issue 2
134-146
EN
Thermus ruber produces (-glucosidase detected in the crude extract of cell proteins. This enzyme exhibits optimum actiivity at 65(C and pH 6,0. The enzyme was stable within a range of pH 5.5 to 8.0 and in 65(C for 60 min. The rate of p-nitrophenol-(-D-glucopyranoside cleavage was higher than that for maltose. With maltotetraose, maltopentaose and maltohexaose, the hydrolysis rate decreased with increasing the molecular weight of the substrate. Our data suggest that the starch converting process could be improved using (-glucosidase from Thermus ruber.
2

Changes in the content of major proteins and selected hormones in the blood serum of piglets during the early postnatal period

100%
Szymeczko R., Kapelanski W., Piotrowska A., Dybala J., Boguslawska-Tryk M., Burlikowska K., Hertig I., Sassek M., Pruszynska-Oszmalek E., Mackowiak P.
Folia Biologica
|
2009
|
vol. 57
|
issue 1-2
97-103
EN
The aim of the study was to determine the changes in the content of major proteins, glucose and selected hormones in the blood of piglets during the first 7 days of neonatal life. The study involved an entire litter of eight newborn piglets of F1 hybrids (Polish White Large x Polish Landrace) delivered from one sow in the second gestation. In blood samples collected directly after parturition (before colostrumintake), in the 12th, 24th and 48th hour and in 7th day of life, the content of total protein and its fractions, glucose concentration and the level of insulin, T3 (total and free), T4 (total and free), leptin, resistin and ghrelin (total and active) was determined. In the blood serum of newborn piglets a low content of total protein, albumins, gamma globulins and a high share of alpha- and beta globulins was found. In the 12th hour of life, after colostrum intake, a significant (P<0.05) increase in the content of total protein, albumins, beta-globulins and a rapid increase of gamma globulins as well as decrease of alpha-globulins level were observed. In the consecutive periods of postnatal life a significant (P<0.05) decrease of total protein, beta- and gamma globulins as well as a steady increase in the content of albumins in the blood serumof piglets was observed. The content of glucose, insulin, leptin, resistin and ghrelin in the blood serum of neonates increased significantly (P<0.05) after colostrum intake. During the successive experimental periods a progressive increase (P<0.05) of glucose and T3 as well as systematic decrease of insulin, T4, ghrelin and resistin in the blood serum was observed as compared to the 12th hour of life.
3

Physiological evaluation of in vitro cultivated plants

100%
Borkowska B.
Biotechnologia
|
2001
|
issue 2
133-136
EN
The aim of the present work was to evaluate the morphological and physiological status of strawberry shoots (cv. Senga Sengana) cultivated in vitro and their subsequent (out of glass vessels) ability to form plantlets with developed autothrophic metabolism. Standard medium recommended by Boxus was supplemented with glucose or sucrose 30 g/l. Biomass production and particular shoot formation were more efficient in the presence of glucose. The capacity of the shoots to form the root system and to develop photosynthetic activity was higher for shoots taken from the glucose-medium than the sucrose containing medium.
4

Using respiratory deficient mutants of Saccharomyces cerevisiae to glucose and xylose cofermentation with Pichia stipitis

88%
Kordowska-Wiatr M., Targonski Z.
Biotechnologia
|
1997
|
issue 4
94-102
EN
One of the main problems limiting the economic production of ethanol from lignocellulosic biomass is D-xylose fermentation. In a medium containing glucose and xylose, it is preferable to achieve conversion with glucose fermenting yeast like S. cerevisiae and xylose fermenting yeast like P. stipitis. In order to resolve this problem, it is better to use respiratory deficient mutants. In this research, respiratory deficient mutant strains S. cerevisiae V30 and Ja(a) were obtained and their ability to ferment glucose in coculture with P. stipitis was investigated. A higher xylose conversion was observed in P. stipitis cultivation with these mutants because of better oxygen conditions than in the culture with native S. cerevisiae. A degree of assimilated xylose did not efficiently increased ethanol yields but on the other hand it increased the production of yeast biomass. Process considerations in relation to the fermentative performances using different strains combinations are discussed.
5

Genetic and epigenetic factors affecting meiosis induction in eukaryotes revealed in Paramecium research

75%
Prajer M.
Folia Biologica
|
2008
|
vol. 56
|
issue 1-2
1-11
EN
This review presents studies of the induction of meiosis undertaken on the ciliate Paramecium, a unicellular model eukaryotic organism. Meiosis in Paramecium, preceding the process of fertilization, appears in starved cells after passing a defined number of divisions (cell generations), starting from the last fertilization. Investigations were performed on clones of cells entering autogamy, a self-fertilization process. Genetic as well as epigenetic factors, i.e. endo- and exogenous factors, affecting the induction of meiosis and changing the duration of the interautogamous interval (IAI), were analyzed. The results show that: (1) Meiosis induction is controlled genetically by the somatic macronucleus. However, besides the nuclear factors, the cytoplasmic protein immaturin also affects this process (HAGA & HIWATASHI 1981); (2) Epigenetic factors, such as non-genetically disturbed cytoskeleton structures and changes in the cell architecture observed in doublet Paramecium cells, exert internal mechanical stress (INGBER 2003), which constitutes the endogenous impulse accelerating meiosis; (3) Mild osmotic stress, acting as an exogenous factor, can initiate the specific MAP kinases signaling pathway resulting in earlier meiosis induction, as in other unicellular eukaryotes (SEET & PAWSON 2004).
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