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Accurate codon recognition by tRNAs is necessary for correct translation of mRNA nucleotide sequence into the protein sequence. Here, different factors contributing to the correct codon reading by tRNAs are reviewed. In particular, the monitoring of codon-anticodon helix geometry by 16S rRNA bases, and the role of tRNA sequence elements and posttranscriptional modifications for modulating codon-anticodon interactions are discussed.
EN
The effect of salicylic acid (SA) applied during the proliferation phase of cell suspension in vitro regeneration system of Medicago sativa L. on cell suspension growth, production of somatic embryos and their following development, germination and conversion were examined. SA is a potent inhibitor of the above processes. It is suggested that endogenous jasmonates are of some importance in regulation of somatic embryogenesis.
EN
Responses to salt stress of diploid wheat Triticum monococcum L., tetraploid wheats T. dicoccum (Schrank) Schubl. and T. durum Desf. cv. Grandur, and hexaploid wheats T. aestivum L. cvs. Begra and Gama were studied. Seeds were germinated on filter paper in Petri dishes, moistened in Hoagland medium containing 0 (control), 10, 20, 50, 100 and 200 mM NaCl. Index of tolerance to salt treatments was calculated on the basis of shoot length. Increase in salt concentration led to decrease in seed germination and inhibition of shoot and root elongation. Hexaploid and diploid wheats were more tolerant to salt stress than tetraploid wheats.
EN
Somatic embryogenesis (SE), resembling zygotic embryogenesis, is a very efficient and fast method of vegetative plant propagation. Somatic embryos, the final products of this process, are immediately or after drying and/ or encapsulation used as artificial seeds. SE efficiency is not only dependent on the quantity, but also on quality of somatic embryos. Although SE has been recorded for species across many genera and from a variety of plant tissues, regeneration of plants from somatic embryos is often a significant problem for some plant species. Many efforts have been made to obtain higher levels of germination and conversion of somatic embryos to plants. This review will focus on the present knowledge about enhancing the vigor of somatic embryos with special attention paid to the effect of plant hormones (gibberellins, ethylene, abscisic acid), germination and conversion of somatic embryos and their influence on the storage reserves content (starch, oligosaccharides), their hydrolytic products (raffinose, sucrose, glucose), and on the activity of hydrolytic enzymes (alpha-amylase).
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