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1

Allozyme variability of putative hybrid swarm population (Pinus mugo Turra x P.sylvestris L.) from Topielisko peat-bog near Zieleniec

100%
Siedlewska A., Pros-Glowacki W.
Genetica Polonica
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1994
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vol. 35
|
issue 4
285-302
EN
The purpose of investigation was to analyse the isoenzymatic variability of the population from Topielisko peat-bog, representing probably a hybrid swarm (Pinus mugo Turra x P.sylvestris L.), and compare the genetic structure of this population with that of P.mugo and P.sylvestris populations as putative parental species.Allozyme variation at 10 enzymatic loci was studied in a group of 300 individuals from the population in Topielisko reservation against variation of 5 P.mungo populations from the Tatra Mts. (2 of which from the mountain peat-bogs) and 3 P.silvestris populations.
2

IRAP and REMAP assessments of genetic similarity in rice

100%
Branco C. J. S., Vieira E. A., Malone G., Kopp M. M., Malone E., Bernardes A., Mistura C. C., Carvalho F. I. F., Oliveira C. A.
Journal of Applied Genetics
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2007
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vol. 48
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issue 2
107-113
EN
Rice is a model genome for cereal research, providing important information about genome structure and evolution. Retrotransposons are common components of grass genomes, showing activity at transcription, translation and integration levels. Their abundance and ability to transpose make them good potential markers. In this study, we used 2 multilocus PCR-based techniques that detect retrotransposon integration events in the genome: IRAP (inter-retrotransposon amplified polymorphism) and REMAP (retrotransposon-microsatellite amplified polymorphism). Markers derived from Tos17, a copia-like endogenous retrotransposon of rice, were used to identify genetic similarity among 51 rice cultivars (Oryza sativa L.). Genetic similarity analysis was performed by means of the Dice coefficient, and dendrograms were developed by using the average linkage distance method. A cophenetic correlation coefficient was also calculated. The clustering techniques revealed a good adjustment between matrices, with correlation coefficients of 0.74 and 0.80, or lower (0.21) but still significant, between IRAP and REMAP-based techniques. Consistent clusters were found for Japanese genotypes, while a subgroup clustered the irrigated Brazilian genotypes.
3

Variability of cathodic peroxidases in sugar beet (Beta vulgaris L.) cultivars

100%
Kolodziejczak M., Krzakowa M.
|
EN
Eighteen varieties of sugar beet (Beta vulgaris L.), originating from various European countries, were compared in respect of peroxidase variability level. They were cultivated in the same experimental plot. The cultivars differed in ploidy level: one variety was tetraploid, three were diploid and 14 varieties were triploid. The cathodic peroxidase system is controlled by four independent genes, of which only one is polymorphic. Consequently, the varieties were characterised by frequencies of 3 allozymes belonging to one locus. Only one variety proved to be fully monomorphic. Genetic similarities between the cultivars were illustrated by a dendrogram (UPGMA) and show different groups of varieties not related to their ploidy level.
4

Genetic relationships among wheat genotypes, as revealed by microsatellite markers and pedigree analysis

100%
Sud S., Bains N. S., Nanda G. S.
Journal of Applied Genetics
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2005
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vol. 46
|
issue 4
375-379
EN
Genetic relationships among 20 elite wheat genotypes were studied using microsatellite markers and pedigree analysis. A total of 93 polymorphic bands were obtained with 25 microsatellite primer pairs. Coefficient of parentage (COP) values were calculated using parentage information at the expansion level of 5. The pedigree-based similarity (mean 0.115, range 0.00?0.53) was lower than the similarity assessed using microsatellite markers (mean 0.70, range 0.47?0.91). Similarity estimates were used to construct dendrograms by using the unweighted pair-group method with arithmetic averages (UPGMA). Clustering of genotypes in respect of marker-based similarity revealed two groups. Genotype PBW442 diverged and appeared as distinct from all other genotypes in both marker-based and pedigree-based analysis. The correlation of COP values with genetic similarity values based on microsatellite markers is low (r = 0.285, p < 0.05). The results indicate a need to develop wheat varieties with a diverse genetic background and to incorporate new variability into the existing wheat gene pool.
5

Genetic diversity of inbred rye lines evaluated by RAPD analysis

88%
Myskow B., Masojc P., Banek-Tabor A., Szolkowski A.
Journal of Applied Genetics
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2001
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vol. 42
|
issue 1
1-14
EN
This study presents an attempt to supply breeders of hybrid rye with more genetic information on inbred lines, using molecular markers. Eighteen polymorphic loci detected by means of the RAPD (Random Amplified Polymorphic DNA) technique and mapped on 2R-7R rye chromosomes, were applied to study genetic similarities among forty inbred lines of rye. The lines were grouped in four main clusters revealed on dendrogram, which was generally consistent with the pedigree data. Mapped RAPD markers were shown to be a useful tool for phenetic studies in rye. Additionally, a system of 20 polymorphic fragments, detected by three primers, was developed for fingerprinting of rye lines. The system of RAPD markers, which was developed in this study, should be helpful in characterisation of rye genetic stocks used for breeding.
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