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  1. 1 Biotechnologia

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  1. 1 2009

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  1. 1 Grajek W.

  2. 1 Sadowska J.

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Analysis of physiological state of single bacterial cell using fluorescent staining methods

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Sadowska J., Grajek W.
Biotechnologia
|
2009
|
issue 4
102-114
EN
The paper reviews fluorescent staining techniques allowing to diagnose the physiological state of bacterial cells. Different staining probes and a manner of their use for analysis of cell viability, membrane potential, membrane integrity, intracellular pH, respiratory activity, amount of nucleic acids, and activity of chosen intracellular enzymes are described. Range of examples of fluorescent staining for monitoring of physiological state of bacteria in natural environment and in biotechnological processes are presented.
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