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1

Localisation of rDNA in the Lupinus genome during the cell cycle

100%
Naganowska B., Zielinska A.
Journal of Applied Genetics
|
2004
|
vol. 45
|
issue 2
189-193
EN
Observations of a specific rDNA locus behaviour during the cell cycle were made by fluorescent in situ hybridisation (FISH) in 12 Lupinus species. Due to the pattern of chromatin de-condensation in that locus, the number of relevant sites in interphase nuclei was twice as high as the number of signals on metaphase chromosomes. The description of successive phases and an attempt of an explanation are given.
2

Nucleic acids in situ hybridization: from ISH to DIRVISH

88%
Maluszynska J.
Biotechnologia
|
1995
|
issue 2
92-101
EN
In situ hybridization (ISH) provides a highly sensitive method for the detection of specific nucleic acid sequances in cells, nuclei and metaphase chromosomes.This technique has been improved continuously since it was first established in 1969.Especially fluorescent in situ hybridization (FISH) rapidly replaced radioactive procedures and became a conventional tool in cytogenetic research.ISH plays an increasingly important role in a variety of reserch areas of medicine, genetics and plant breding.It has been succesfully applied for chromosome or chromosome fragments identification, chromosomal abormlities detection, gene mapping or specific DNA sequences localization.
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