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Strains of Y.lipolytica were flocculated in the presence of two additives either bentonite or chitosan.The effects of the flocculant doses and the pH on the yeast flocculation were studied.Stability of cell flocs and their capability to citric acid production was examined in shaker flask and stirred bioreactoe cultures.The optimal flocculent dases corresponding to the maximal reduction of turbidity of cell suspensions (%RT) in the production medium were in the range of 10-110 mg chitosan/g biomass and 30-80 mg bentonite/g biomass.Flocculated Y.lipolytica produced less citric acid and the volumetric citric acid productivity was lower as compared to free call cultures.
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