Search results

1

Analysis of oocyte quality in recombinant inbred mouse strains developed from KE and CBA strains that differ in fertilization efficiency

100%

Wabik-Sliz B., Golas A., Krzanowska H.

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EN

Recombinant inbred (RI) mouse strains were developed from reciprocal crosses between two inbred strains differing in the proportion of fertilized ova (CBA, 100%; KE, 77%), to analyse the underlying factors. A correlation (r = 0.83, P < 0.01) between fertilization efficiency within 22 RI strains and after mating RI females with KE males proved that oocyte quality was involved. The following oocyte parameters were analysed in RI and progenitor strains: time of meiotic maturation, rapidity of enzymatic removal of egg investments, and proportion of fertilized ova with supplementary spermatozoa in the perivitelline space. Among the RI strains, high incidence of supplementary spermatozoa was correlated with lower efficiency of fertilization (r = ?0.58, P < 0.05) and with slow meiotic maturation (r = ?64, P < 0.01), suggesting that delayed maturation may affect oocyte ability of being fertilized by the first penetrating spermatozoon. However, significant correlations were also found between characters which coexist within the progenitor strains, but are not likely to be physiologically related; this suggests that RI strains have inherited large blocks of progenitor genomes, not disrupted by recombination. The strain distribution pattern (SDP) of the analysed traits revealed CBA-like, KE-like, and intermediate phenotypes, indicating that they are polygenically determined. No linkages were found between the studied traits and 12 enzymatic markers. However, the SDP for fertilization efficiency showed a preponderance of non-matching strains (15/19) in relation to agouti locus; the known instability of this chromosome region makes it possible that a putative linkage was disrupted by recombination when RI strains were created.

2

Molecular basis of and

88%

KurpiszMaciej M. M.

Postępy Higieny i Medycyny Doświadczalnej

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1993

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vol. 47

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issue 1

19-31

EN

There has been described molecular events towards preparation of for as (process known to be characterized by increased mobility of membrane components on sperm surface) and acrosomal reaction (fusion of spermatozoon plasmalemma with outer acrosomal membrane with following destruction of acrosomal compartment and enzyme release). These have been also described hypotheses concerning activity of exposed during acrosomal reaction receptors for and especially located on egg's . The role of acrosomal enzymes in penetration of zona pellucida by sperm as well as inhibition of enzymatic action were discussed in aspect of contraceptive potential.

3

Early stages of embryogenesis after fertilization in vitro condition

88%

Dziedzic E., Lech W., Malodobry M.

Biotechnologia

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2001

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issue 2

54-56

EN

Ovules of plum cultivars (Sweet Common Prune, Herman, Gilbert, Sanctus Hubertus, Cacanska Rodna, Cacanska Najbolia) were pollinated with pollen of cv. Stanley on the White?a medium with addition of 15% sucrose. The nucelluses excised from the fertilized ovules were cultivated on Norstog medium with addition of 3,4% sucrose. The process of fertilization after pollination in the in vitro condition was proved on the basis of the paraffin cross?sections: the degeneration of synergid after entering the pollen tube, the fertilization of egg cell were confirmed. The microscopic observations showed the swelling of micropylar part of nucellus. The paraffin cross-sections showed a many-layer kalotka (peculiar morphological structure) and swelled cells of nucellus among which the embryo sac was observed. The presence of embryo without endosperm or the presence of endosperm without embryo in the embryo sac was noted.

4

Efficiency of nitrogen and phosphorus utilization in progenies of factorial crosses between European and exotic cultivars of spring barley (Hordeum vulgare L.)

63%

Gorny A. G., Ratajczak D.

Journal of Applied Genetics

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2008

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vol. 49

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issue 4

349-355

EN

Previous observations suggested that some landraces, primitive cultivars and other barleys originating from the harsh conditions of the Middle East, North Africa and Tibet might serve as interesting sources of adaptation to low-input agriculture. This opportunity was verified in field experiments performed under reduced rates of nitrogen (N) and phosphorus (P) fertilization. Genetic variation in major components of the efficiency of N and P utilization was examined among F2 and F3 generations of crosses between four European female and seven exotic male lines of spring barley. Preponderance of general combining ability effects was found for most efficiency components, suggesting that the characters are mainly controlled by additive gene action. Non-additive effects appeared to be less or non-significant. Under reduced fertilization, hybrid progenies of the Syrian cv. M.Dingo/D.Alla 106 and the Moroccan cv. Moroc 9-75 exhibit an enhanced yielding and the highest capacity to utilize N and P efficiently in grain mass formation. The results proved that the exotic barleys appear to be particularly useful for breeding programs aiming at improved barley adaptation to less favourable fertilization regimes.

Refine search results

2 Journal of Applied Genetics

1 Biotechnologia

1 Postępy Higieny i Medycyny Doświadczalnej

1 2008

1 2005

1 2001

1 1993

1 Dziedzic E.

1 Golas A.

1 Gorny A. G.

1 Krzanowska H.

1 KurpiszMaciej M. M.

1 Lech W.

1 Malodobry M.

1 Ratajczak D.

1 Wabik-Sliz B.

Analysis of oocyte quality in recombinant inbred mouse strains developed from KE and CBA strains that differ in fertilization efficiency

Molecular basis of and

Early stages of embryogenesis after fertilization in vitro condition

Efficiency of nitrogen and phosphorus utilization in progenies of factorial crosses between European and exotic cultivars of spring barley (Hordeum vulgare L.)