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1

Reproductive performance of a transgenic boar carrying the bovine growth hormone gene (bGH)

100%
Rejduch B., Slota E., Sysa P., Rozycki M.
Journal of Applied Genetics
|
2002
|
vol. 43
|
issue 3
337-341
EN
The aim of this study was to obtain information on the possible influence of the bovine growth hormone gene (bGH) on gametogenesis and reproductive parameters of a 2-year-old Polish Landrace transgenic boar. The bGH gene construct had been introduced into the zygote of the boar with the use of the microinjection technique. On the basis of the available documentation we established that the fertility of the investigated transgenic (bGH) boar was low in comparison with other animals of the Polish Landrace breed, with a poor libido, ineffective matings and, on average, 3 live piglets less per litter. Samples of testis tissue from the boar were obtained after castration. In total, we observed 100 spermatocytes and all of them had normally paired bivalents. It is possible that the boar?s lower fertility was caused by some, as yet unknown factor.
2

Diversity of diploid androgenic Brussels sprout plants of R0 and R1 generations

100%
Kaminski P., Dyki B., Krzyzanowska D., Gorecka K.
Journal of Applied Genetics
|
2005
|
vol. 46
|
issue 1
25-33
EN
Androgenic Brussels sprout plants were produced by the use of anther culture from the donor cultivar 'Philemon F1'. A total of 96 plants obtained from 20 androgenic R0 genotypes assigned as diploids were evaluated both in the generative and vegetative stage, in respect of their morphological characters: mean plant height; leaf size, colour and waxiness; leaf blade shape, blistering and attitude; number of sprouts; as well as their self-incompatibility and fertility. Androgenic R0 plants derived from each of the 20 embryos were highly diversified and differed from the donor in one or more morphological traits in the vegetative stage. Evaluated populations also varied in fertility and self-incompatibility. Six androgenic genotypes that set a sufficient amount of seeds of the R1 generation and 'Philemon F1' were evaluated in the field in respect of plant height, total and marketable yield per plot, shape of stem with sprouts, shape and density of sprouts, and spacing between sprouts. Only four diploid R0 and R1 populations may have some value for further breeding, as they are characterised by good vigour, high or medium ability for sprout generation, and sufficient fertility.
3

Chromosomal aberrations diagnosed in farm animals under cytogenetic control at the National Research Institute of Animal Production

100%
Slota E., Danielak-Czech B., Rejduch B., Kozubska-Sobocinska A.
Biotechnologia
|
1999
|
issue 4
171-178
EN
Cytogenetic studies, carried out between 1978 - 1998 at the National Research Institute of Animal Production resulted in identification of several chromosomal aberrations as follows: chromosomal chimerism XX/XY in cattle, sheep and horses, Robertsonian translocations and sex chromosome trisomy in cattle, reciprocal translocations in pigs and sheep, pericentric inversion in pigs. The influence of Robertsonian and reciprocal translocations on fertility of aberration carriers was evaluated and economic losses in pigs due to decreased fertility were calculated. In the light of these results it is advisable to continue cytogenetic control of farm animals in Poland, mainly males being used in reproduction.
4

Some morphological and phenological traits and fertility of lines of artificial winter oilseed rape originated from male-sterile plants (Barssica napus var.olifera L.)

100%
Wojciechowski A.
Genetica Polonica
|
1993
|
vol. 34
|
issue 4
317-325
5

Morphology and fertility of spontaneous octoploid of white clover (Trifolium repens L.)

100%
Kazimierska E. M.
Journal of Applied Genetics
|
1995
|
vol. 36
|
issue 4
341-352
EN
In the progeny of a hybrid obtaned after crossing local Polish and local Bulgarian white clover genortypes one octoploid (2n=64) plants was selected.In view of difficulties to obtain seeds, the octoploid plant was vegetatively propagated.The progeny of the octoploid obtained as a results of self-and-cross-pollination of cloned plants was examined and compared to related tetraploids with respect to morphological characters, fertility and embryo sac structure.Vegetative and generative organs of the octoploid were slightly larger than those of tetraploids.The number of flowers per head was larger in the octoploid than in tetraploid plants.The spontaneous octoploid appeared to have a low fertility after both cross- and self-pollination.It was most probably caused by low polen viability, by decline of megasporocyte and megagametocyte in the process of ontogenesis and by smaller ovule number per ovary.Seeds of octoploids were partially under developed and only 23.85% of them gave rise to seedlings.
6

Cytogenetics, embryology and fertility of red clovere (Trifolium pratense L.) with short flowers and anthocyanin spots on the leaves

88%
Kazimierski T., Kazimierska E. M.
Journal of Applied Genetics
|
1995
|
vol. 36
|
issue 4
353-362
EN
Anthocyanin spot on the leaves of red clover is inherited indepently of light spot and uniform leaf colour.The gene determining anthocyanin spot is probably localized on the other chromosomes than the gene determining light spot on the leaves.A short flower tube is also determined by a single recessive gene of a pleiotropic action.It stimultaneously causes reduction of pistil style, thickening of the endithecium layer in anthers - entails their indehiscence, pollen grain-agglutination and often deformation, no division of part of pollen grains into a vegetative and generative cells as well as germination of a single grains in pollen sacks.The changes in the structure of endothecium and pollen grains cause almost complete male and female infertility of plants with short flower tubes.
7

Hybrids and amphiploids of Aegilops ovata L. with Secale cereale L.: production, morphology and fertility

88%
Wojciechowska B., Pudelska H.
Journal of Applied Genetics
|
2002
|
vol. 43
|
issue 4
415-421
EN
Hybrids (2n = 3x = 21) between Aegilops ovata and Secale cereale were produced via embryo rescue. Most hybrid morphological traits were intremediate between parents, the plants grew vigorously but were completely sterile. The average frequency of chromosome associations at metaphase I was 19.57-20.19 univalents and 0.40-0.86 rod bivalents. The fertility of the hybrids was restored by doubling their chromosome numbers by colchicine treatment and in vitro propagation. Selfed seeds were obtained from colchicine-doubled sectors and some callus regenerates. The seeds were mostly well formed and developed vigorous plants without embryo culture. Colchicine- and callus-derived amphiploids (2n = 6x = 42) resembled the F1 plants in overall morphology, but showed a lower tillering ability, broader leaves, thicker culms and larger spikes.
8

Meiosis and fertility in spontaneous tetraploids of Festuca pratensis (Huds.)

88%
Wisniewska H.
Journal of Applied Genetics
|
1995
|
vol. 36
|
issue 3
205-213
9

Development of new cytoplasmic-genetic male-sterile lines in pigeonpea from crosses between Cajanus cajan (L.) Millsp.and C. scarabaeoides (L.) Thouars

75%
Kalaimagal T., Muthaiah A., Rajarathinam S., Malini S., Nadarajan N., Pechiammal I.
Journal of Applied Genetics
|
2008
|
vol. 49
|
issue 3
221-227
EN
Exploitation of hybrid vigour is quite possible in cross-pollinated crops. However, pigeonpea is a grain legume crop with a moderate level of cross-pollination (20?70%), which is mainly aided by insect pollinators. As a first step, hybrids based on genetic male sterility (GMS) were developed in pigeonpea, but the hybrid seed production technique is not farmer-friendly, because in the hybrid seed production plot 50% of the population, which are male-fertile in the female rows, have to be eliminated in time before contamination. This requires skilled labour and is a time-consuming process, which increases the cost of hybrid seed production. Therefore, the objective of this study was to develop cytoplasmic-genetic male-sterile (CGMS) lines in pigeonpea through wide hybridization, which would be very suitable for hybrid seed production. Two CGMS lines, viz. CORG 990052 A and CORG 990047, were developed by interspecific hybridization of Cajanus cajan and C. scarabaeoides. Restorers were identified and three CGMS-based pigeonpea hybrids were developed. The hybrid COPH 3 is found to be promising in Tamil Nadu State, India.
10

Meiosis and fertility of reciprocal triploid hybrids of Lolium multiflorum with Festuca pratensis

75%
Naganowska B., Zwierzykowski Z. Z., Zwierzykowski Z., Zwierzykowska Z. E., Zwierzykowska E.
Journal of Applied Genetics
|
2001
|
vol. 42
|
issue 3
247-255
EN
Abstract. Diploid and tetraploid forms of Lolium multiflorum and Festuca pratensis were crossed under controlled conditions and after embryo rescue all four combinations of autoallotriploid hybrids were obtained. Male and female fertility and chromosome pairing at metaphase I of meiosis were studied in several plants from each hybrid combination. The hybrids with two genomes of L. multiflorum and one of F. pratensis (genomic formulae LmLmFp and FpLmLm) were male and female fertile while the hybrids with two genomes of F. pratensis and one of L. multiflorum had a reduced fertility (FpFpLm) or were completely sterile (LmFpFp). Chromosome pairing at metaphase I varied among hybrid combinations depending on their genomic composition. LmLmFp and FpLmLm hybrids had similar patterns of pairing (1.83I + 5.29II + 2.85III and 2.22I + 5.22II + 2.75III, respectively) but they differed from those of FpFpLm (3.65I + 4.65II + 2.68III) and especially from LmFpFp (4.78I + 5.87II + 1.49III). Conventional analysis of meiosis failed to explain the differences in chromosome behaviour and fertility/sterility levels between the autoallotriploid hybrids with two Lolium or two Festuca genomes.
11

Evaluation of the effect of centromeric heterochromatin polymorphism on pig fertility

75%
Kozubska-Sobocinska A., Danielak-Czech B., Slota E., Rejduch B.
Journal of Applied Genetics
|
1996
|
vol. 37
|
issue 3
293-298
EN
C-banding identified centromeric heterochromatin polymorphism, most often located in a pair no 16, was a basis for selecting animals for the experiment.The aim of the experiment was to assess the impact of centromeric heterochromatin polymorphism on pig fertility, expressed by litter size.The research included the first two litters obtained from 30 pairs of parent with different genotypes as regards the parameter under study.A statistical analysis of the number of offspring from different mating types showed no significant differences between the experimental groups of animals.The results obtaned did not confirm suggested correlation between polymorphism of the centromeric heterochromatin regons and pig fertility, estimated by mean size.Thus the polymorphism cannot be regarded as a selection criterion.
12

Expression of duplicated segments of satellited chromosomes in spring barley (Hordeum vulgare L.)

75%
Sodkiewicz T.
Genetica Polonica
|
1993
|
vol. 34
|
issue 3
223-236
EN
Barley lines with different size of of the short arms of the chromosome 6 and 7 were investigated under greenhouse and field conditions. The objective of the study were morphological features plant fertility and their dependence on the size of a duplicated segment of chromosome and on conditions of plant growth and development. Generally, duplicated lines under field conditions were observed to have a reduced stem lenght, spike size and spike fertility. The largest reduction of all the studied morphological features occurred in the line 9D with the largest duplicated segments, wheras the smallest differences compared to the normal karyotype were in the line 7D with the smallest duplicated segments. It was found that a bigger duplicated segment made large differences in parameters of morphological features manifested under various growth conditions (particularly in plant height). The results indicate that fertility and investigated morpholofgical features of plants with duplicated chromosome segments are affected not only by the absolute amount of genetic material, but also by the structure of translocated chromosomes building up individual karyotypes.
13

The course of meiosis, pollen fertility and seed setting in barley lines with duplications in chromosomes 6 and 7

75%
Sodkiewicz T., Sodkiewicz W.
Genetica Polonica
|
1994
|
vol. 35
|
issue 4
231-240
EN
The aim of the present study was to find the reasons a low fertility of plants with duplicated segments of the short arms of satellited chromosomes.An attempt was made to determine the influence of duplicated segments of different size on the course of meiotic division, pollen stainability and viability as well as to evaluate the influence of these traits on seed setting in barley lines carrying duplications.Material for the study were spring barley (Hordeum vulgaris) lines, derived from the cv.Bonus with structural changes in chromosomes 6 and 7.It was found that percentage of bivalents formed in the course of meiotic division is correlated with the size of a duplicated sector.The formation of quadrivalent chromosome linkages in the course of meiosis was observed in the lines with duplications, and the number of quadrivalents per cell increased with an enlarged size of a duplicated chromosome sector.Seed setting in the lines carrying duplications may be negatively affected by a small amount of pollen grains in the anthers.Per cent of fertile flowers in the group of lines with duplications was not directly correlated either with per cent of viable pollen grains or with the size of a duplicated sector.
14

Production and characterization of amphiploids of Aegilops kotschyi and Ae. biuncialis with Secale cereale, and of backcross hybrids of Ae. biuncialis ? S. cereale amphiploids with 2x and 4x S. cereale

75%
Wojciechowska B., Pudelska H.
Journal of Applied Genetics
|
2005
|
vol. 46
|
issue 2
157-161
EN
Amphiploids (2n = 6x = 42) of Ae. kotschyi and Ae. biuncialis with self-compatible S. cereale were produced from F1 sterile hybrids (2n = 3x = 21) through colchicine treatment and callus tissue regeneration. The amphiploids resembled the F1 plants in overall morphology, but were larger in all respects and self-fertile. The spikelets consisted mostly of 3 well-developed florets. Selfed seeds were obtained from some colchicine-doubled sectors and callus regenerates. Most of the produced seeds were well developed. Backcrosses between amphiploids and rye (2x and 4x) resulted in obtaining (Ae. biuncialis ? S. cereale amphiploid) ? S. cereale hybrids via embryo culture. The BC1 plants (2n = 4x = 28 and 2n = 5x = 35, respectively) were phenotypically intermediate between the parents and vigorous in vegetative growth. Some seeds were obtained only from the 35-chromosome BC1 hybrids.
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