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1

Optimization of simultaneous saccharification and fermentation of the native starch by using response surface method

100%
Bialas W., Wojciechowska D., Szymanowska D., Grajek W.
Biotechnologia
|
2009
|
issue 4
183-199
EN
Ethanol production, by a simultaneous saccharification and fermentation process (SSF) of native starch from corn flour, has been performed by Saccharomyces cerevisiae (Ethanol Red) and granular starch hydrolyzing enzyme (STARGEN 001). The quantitative effects of mash concentration, enzyme dose and pH were investigated by the use a Box?Wilson central composite design protocol. It was found that for native corn starch, maximum ethanol concentration of 110,36 g/l was obtained using a mash concentration of 25%, which resulted in ethanol yield of 85,71%. The optimum conditions for the above yield were found for the enzyme dose of 2,05 ml/kg and pH of 5.0. These results indicated that by using the central composite design, it is possible to determine accurate values of the fermentation parameters for maximum ethanol production.
2

Ethanol production in membrane bioreactor

100%
Gryta M., Morawski A. W.
Biotechnologia
|
1998
|
issue 3
147-157
EN
A membrane bioreactor for alcohol fermentation is presented in this paper. Membrane distillation process was used for the removal of ethanol. The ethanol fluxes through the polypropylene membrane were approximately 2-3.5 kg EtOH/m2d. The yield of ethanol equals 0.45-0.51 g EtOH/g sugar was obtained.
3

Role of plasma membrane in ethanol tolerance of yeasts

100%
Grajek W., Walkowiak-Tomczak D.
Biotechnologia
|
1993
|
issue 3
157-165
EN
Tolerance of yeast to high ethanol concentration depends on cell membrane lipid composition. High content of unsaturated fatty acids and sterols increase the cell structure stability and viability as well as the fermentation activity, of yeasts. The cultivation of microorganisms at elevated temperatures and high increase the degree and as a consequence the cell sensibility to stress. Natural tolerance of yeasts to ethanol can be improved by technological means. It can be done by supplementation of fermentation broth with a source of unsaturated lipids, and, alternatively, by medium aeration to stimulate .
4

Bioethanol production from cellulose raw material

100%
Leja K., Lewandowicz G., Grajek W.
Biotechnologia
|
2009
|
issue 4
88-101
EN
Lignocellulose is a potential non-food raw material for production of bioethanol. Its use gives the world economy a chance to be independent from petrochemical industry. In the last decade, interest in bioethanol production from waste raw materials increased. However, production processes require more research in order to optimize production conditions and decrease its cost. In each production stage, there appear various problems, for example, a selection of hydrolysis enzyme and microorganisms leading to fermentation. Probably, in the near future, the productivity of bioethanol from waste materials will improve.
5

Genetic modification of microorganisms for ethanol production from hemicellulosic hydrolyzates

100%
Nowak J.
Biotechnologia
|
1998
|
issue 3
89-97
EN
The efficient and complete conversion of lignocellulosic hydrolysates to ethanol as a result of fermentation processes makes possible the production of a bio-fuel competitive with petroleum. Traditional organisms used in ethanol fermentation, e.g. Saccharomyces cerevisise and Zymomonas mobilis, are unable to ferment pentose sugars. To overcome this problem genetic modifications of S. cerevisiae, Z. mobilis and E. coli were performed in order to produce ethanol directly from xylose and arabinose. The performance of the recombinant strains is encouraging: especially over the last 3 years the progress has been rapid. There is a possibility that strains of these three species may find a separate application in the fermentation of specific plant biomasses.
6

Biotechnology in Polish food science ? achievement and perspectives

100%
Grajek W.
Biotechnologia
|
2002
|
issue 4
41-55
EN
Polish food biotechnology science has achieved many successes in past years. Many new technologies have been introduced into food industry and many new products have been offered to consumers. The research related to food biotechnology is concentrated on agricultural universities, technical universities and on branch institutes. Although traditional biotechnology in Poland appears to be well developed, there are many groups working on transgenic plants and animals. In general, only few modifications concern the features important for food applications.
7

Microbial production of ethanol from lignocellulosic wastes

100%
Szczodrak J., Fiedoruk J.
Biotechnologia
|
1993
|
issue 3
145-156
EN
Current trends in the production of ethanol from lingocellulosics are reviewed. Particular emphasis is laid on the preytreatment of the lignocellulose materials and their simultaneous saccharfication and fermentation to ethyl alcohol.
8

Energy-saving and by-products-free production of ethanol from granular corn starch

100%
Szymanowska D., Grajek W.
Biotechnologia
|
2011
|
issue 1
85-91
EN
Intense development of the transport sector and a rise in the prices of fossil fuels boost the demand for fuels from alternative sources of energy, including biofuels. Thus, new energy-efficient technologies of fuel production from renewable resources are being developed. Accordingly, we attempted to create a technology of bioethanol production by simultaneous enzymatic hydrolysis and fermentation of native corn starch. In our work we used commercially available STARGEN 001 enzymatic preparation for native corn starch hydrolyzis, and yeast Saccharomyces cerevisiae strain Red Star Ethanol Red for it's fermentation. A major part of the research was on developing a fermentation technology with repeated full recirculation of stillage liquid fraction. It has been shown that a completely effluentless ethanol production with separation of stillage solid fraction and using the liquid fraction to prepare the next portion of corn mash is possible. Thus the process can proceed for several months without any effluent.
9

Investigation of influence of solid substrate mixing on growth mycelium Pleurotus ostreatus

88%
Kopinski L.
Biotechnologia
|
1999
|
issue 4
143-150
EN
The influence of mixing cut straw on the growth of Pleurotus ostreatus in a rotating drum bioreactor was investigated. Higher concentration of mycelium was determined for low rotation (0,07-0,1 rph) than without rotation of bioreactor. Decrease in concentration of mycelium was observed for increasing rotations and the growth was stopped at 4,8 rph.
10

Batch propionic acid fermentation using propionibacterium sp. immobilized in different supports

88%
Czaczyk K., Trojanowska K.
|
|
issue 1
188-198
EN
Propionibacterium freudenreichii subsp. shermanii 1 and 4 were immobilized in the living state in 2, 3 and 4% alginate gels, in 2, 4 and 6% carrageenan gels, in 2, 4 and 6% carrageenan/locust bean gum gels and on ceramic support. Ammonia consumption, glucose utilization, production of propionic and acetic acids, biosynthesis of vitamin B12 and cell release rate were examined. A significant increase of productivity of propionic acid and decrease of biosynthesis of vitamin B12 using immobilized cells were observed. The best results were obtained in the fermentation with strains immobilized in 4% alginate gel, when applied for the third time. In this case, production of propionic acid was 50% higher in comparison with free cells and biosynthesis of vitamin B12 was lower or the same as in the control.
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