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Effect of Protein Extract of Mangifera indica Pollen on the Erythrocytes (Red Blood Cell) of Albino Rats

100%

Ezebo R. O., Omumuabuike J. N., Esimai B. G., Ilouno E. E., Obieze O. U., Obienyem J. N., Uba C. A.

World News of Natural Sciences

2021

vol. 36

114-124

This study was carried out to show the effect of the protein extract of Mangifera indica on the red blood cell (erythrocytes) of albino rats. The Mangifera protein was extracted using 500ml of 0.02m (20mM) phosphate- buffered saline (PBS), at pH 7.4, in a large beaker (stirred with a magnetic stirrer for 3 hours at room temperature). The crude extract was saturated to 60% by adding solid ammonium sulphate under constant gentle attiring, and then stored in a refrigerator for 6 hours. Sixteen male albino rats obtained from animal house in Faculty of Biological Science, University of Nigeria Nsukka was used for the study. They were divided into two groups of eight. One group was labeled the experimental group and the other control. The extract was administered to the experimental rats intra nasally for a period of seven (7) days. Data were expressed as mean ± standard error. Means were separated using Duncan’s New Multiple Range Test (DNMRT). Blood samples were collected via the orbital plexus of rats to determine the effect of the extract on red blood cell (erythrocytes). The present study demonstrated that the extracted pollen of Mangifera indica had no allergic effect on ratsand so would need to be further investigated.

In-vivo effects of nociceptin and its structural analogue [Orn9] nociceptin on the antioxidant status of rat blood and liver after carrageenan-induced paw inflammation

84%

Petrov L., Tzvetanova E., Pavlova A., Alexandrova A., Zamfirova R., Kirkova M., Todorov S.

Open Medicine

2010

vol. 5

issue 1

123-131

The production of reactive oxygen species (ROS) in cells is well balanced with their elimination by the antioxidant defence system. This balance is essential for maintenance of physiological conditions, and its disturbance (oxidative stress) has been suggested as a potential pathogenic mechanism in a variety of diseases, accompanied by inflammation. In this study, the in-vivo effects of nociceptin (N/OFQ(1–13)NH2) and its structure analogue [Orn9]N/OFQ(1–13)NH2 were studied on markers of oxidative stress in erythrocytes and liver of rats 4 hours after subplantar administration of carrageenan (CG) (1%, 100 µl) in the right hind paw. A considerable inflammatory oedema of the paw was observed. CG did not change blood haemoglobin content, hematocrit value, glutathione level and antioxidant enzyme activities in the erythrocytes, but there was an increase in lipid peroxidation. In liver, CG-induced imbalance was manifested by an increase in lipid peroxidation and a decrease in glutathione level. Both peptides (20 µg, i.p.), when administered alone, had no effect on all parameters tested. When either [Orn9]N/OFQ(1–13)NH2 or N/OFQ(1–13)NH2 was injected simultaneously with CG or 15 minutes before it, they did not affect the CG-induced changes in the antioxidant status of the erythrocytes and liver. Our results suggest that the peptides tested did not play a role in the free radical processes that accompany CG-induced paw inflammation.

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1 Open Medicine

1 World News of Natural Sciences

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1 Alexandrova A.

1 Esimai B. G.

1 Ezebo R. O.

1 Ilouno E. E.

1 Kirkova M.

1 Obienyem J. N.

1 Obieze O. U.

1 Omumuabuike J. N.

1 Pavlova A.

1 Petrov L.

1 Todorov S.

1 Tzvetanova E.

1 Uba C. A.

1 Zamfirova R.

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Effect of Protein Extract of Mangifera indica Pollen on the Erythrocytes (Red Blood Cell) of Albino Rats

In-vivo effects of nociceptin and its structural analogue [Orn9] nociceptin on the antioxidant status of rat blood and liver after carrageenan-induced paw inflammation