Full-text resources of PSJD and other databases are now available in the new Library of Science.
Visit https://bibliotekanauki.pl
Preferences help
Polish version English version Language
enabled [disable] Abstract
Number of results

Results found: 4

Number of results on page
first rewind previous Page / 1 next fast forward last

Search results

Search:
in the keywords:  EXPLANT
help Sort By:

help Limit search:
first rewind previous Page / 1 next fast forward last
1

Topophysis in adventitious shoots regeneration in vitro in chrysanthemum

100%
Zalewska M., Miler N.
Biotechnologia
|
2010
|
issue 2
89-95
EN
Success of breeding programmes using adventitious shoots technique often depends on the regeneration efficiency. The aim of this study was to investigate the influence of topophysical position of explants on the efficiency of adventitious shoots regeneratio . Uniform, single shoots of chrysanthemum 'Satinbleu' propagated in vitro were divided equally into three topophysical zones: distal, central and proximal. Two leaves and two internodes were isolated from each zone. The explants were cultured for 12 weeks on MS medium supplemented with 0.6 mg l-1 BAP and 2.0 mg l-1 IAA. Due to the highest adventitious shoots regeneration capability and the earliest formation of the shoots, the most suitable explants for breeding programmes for chrysanthemum are internodes excised from distal part of in vitro plantlet.
2

Micropropagation of Miscanthus x giganteus (Greef i Deu.) from inflorescence explants

100%
Glowacka K., Zenkteler M., Jezowski S.
Biotechnologia
|
2004
|
issue 2
251-259
EN
2 mm explants of immature inflorescences (0.5-3) produced calluses on MS medium with o.5 mg x 1 ?1 BAP and 2.5 mg x 1-1 2.4-D. The surface sterilization in 10% sodium hypochloride for 20 min was successful in 74%. After 20 days of incubation, the surfaces of all explants were covered by callus. After 2 months of culture, from most of calluses embryo-like structures developed. The highest frequency of fully regenerated plants was observed on MS medium supplemented with 1-2 mg x l-1 BAP. The regeneration rate of the cultured calluses for the first two weeks in 16 h day fotoperiod was twice as high as the one under a continuous light. Only two out of 6 thousand cultured mature spikelets produced calluses from which 574 plants were obtained after multiplication. Most of the plants obtained from the explants representing immature as mature inflorescences survived the transfer into soil in the greenhouses.
3

Formation of adventitious bulblets in vitro on scale explants in Hippeastrum x chmielii Chm.

88%
Witomska M., Ilczuk A.
Biotechnologia
|
2004
|
issue 2
199-205
EN
Trials on micropropagation of a new interspecific hybrid Hippeastrum x chmielii are being carried out in the Department of Ornamental Plants at the Warsaw Agricultural University. In 2002-2003, an effect of BA on bulblet differentiation from explants of different size was tested. Initial material consisted of bulblets (5-6 mm of diameter, mean fresh weight 0,164 g) produced in vitro on MS medium without growth regulators. The bulblets were cut into halves or quarters along the main axis and placed polarly or apolarly on MS medium without growth regulators or with BA 1, 2, 4 or 8 mg?dm-3. Regeneration proceeded at 24oC under white, fluorescent light (24 mumol?m-2?s-1) for 16 hours and 8 hours of darkness. On the explants with basal part in the medium, the cytokinin limited a number of bulblets regenerated on both types of explants. The bulbs cut into quarters formed more bulblets than those cut in halves. When the explants were placed apicaly, the numbers of regenerated bulblets were similar in all the treatments. Fresh weight of bulblets regenerated on smaller explants (1/4 of a bulb) was higher when explants were placed polarly. Polarity did not affect the weight of bulblets produced on larger explants.
4

In vitro culture of Gentiana kurroo (Royle)

75%
Fiuk A., Rajkiewicz M., Rybczynski J. J.
Biotechnologia
|
2003
|
issue 3
267-274
EN
The aim of the presented paper is to show the present status of in vitro studies of Gentiana kurroo (Royle). Experimental material originated from seedlings, organs of regenerants, callus tissue, cell suspensions and isolated protoplasts. Two years of experiments proved very high morphological potential of plant material originated from various system of cultures. The mostly used media were those based on Murashige and Skoog medium, but for protoplast cultures, Kao and Michaluk medium appeared to be most useful. Numerous plant growth regulators with various concentrations and combinations controled plant regeneration of studied gentiana. Plant regeneration via somatic embriogenesis was observed in the majority of the studied culture systems.
first rewind previous Page / 1 next fast forward last
JavaScript is turned off in your web browser. Turn it on to take full advantage of this site, then refresh the page.