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New data on the karyotype structure of five species of the subfamily Eurytominae are escribed, including the following species: Eurytoma rosae (2n=20), E. robusta (n=7, 2n=14), E. serratulae (n=6, 2n=12), E. compressa (2n=10) and Sycophila biguttata (2n=18). 2n=10, 12 and 14 are reported for the first time for the Eurytominae, in which only 2n=20 and 18 have been previously found. The taxonomic and phylogenetic implications of the new chromo- somal information are discussed. Karyotypes containing ten subtelo/acrocentric chromosomes which gradually decrease in size can be considered as initial for the Eurytominae. Eurytoma robusta + (E. serratulae + E. compressa) form a particular clade where all branches are well-supported by morphological, biological and chromosomal a
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