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1

Epithelial cell cultures as a model system to study the transepithelial transport

100%

Grajek W., Olejnik A., Staniaszek K.

Biotechnologia

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2006

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issue 2

148-165

EN

Epithelial cell lines are commonly accepted model system to determine intestinal permeability and transepithelial transport. The application of Caco-2 human epithelial cell culture can be used to study bioavailability of drug and food compounds. This culture grows on porous membranes and forms a monolayer of well differentiated cells joined by intracellular tight junctions. This system provides selective barrier to study passive and active transport of different molecules. The Caco-2 cells posses some enzymes and transport systems similar to intestinal epithelium in vivo, however, these proteins are expressed to a lesser extent compared to normal enterocytes. In this paper, the advantages and limitations of Caco-2 model are discussed.

2

Processes involved in the repair of injured airway epithelia

75%

Tesfaigzi Y.

Archivum Immunologiae et Therapiae Experimentalis

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2003

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vol. 51

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issue 5

283-288

EN

Recent studies have uncovered many aspects of the repair processes that follow airway epithelial injury. Although the repair process has common elements among various epithelia, such as the ones lining the airways, skin, and gut, there are differences based on their diverse functions. Whenever possible, similarities are pointed out that could help researchers further investigate their application to airway epithelia, although it would be beyond the scope of this review to cover the processes that may occur during the repair of all types of epithelia. In general, five major steps are involved in the recovery of airway epithelia from injury: 1) epithelial cells migrate to cover denuded areas within minutes, and certain proteins, such as the trefoil factor family proteins, are crucial to this process; 2) epithelial cells start to proliferate in order to replace injured cells and to differentiate to establish squamous or mucous cell metaplasia; 3) because more epithelial cells are present after proliferation, some of the cells must be discarded to restore the epithelium to the original condition; 4) once the cell numbers have been reduced to those found in unexposed individuals, the normal proportions of cell types are restored; 5) finally, studies from exposures of rats to ozone show that epithelial cells can adapt and develop a memory of the chronic exposure to which they were exposed. This adaptation allows the epithelium to respond quickly, thus minimizing further injury. Although the molecular mechanisms involved in these major steps of the recovery process are largely unknown, disruption of these steps clearly causes the permanent changes observed in diseases such as asthma, chronic bronchitis, and cancer; therefore, extensive research in these areas may provide ideas for novel therapies.

3

Foot and mouth disease (FMD) - actual diagnostic methods

75%

Paprocka G., Kesy A., Niedbalski W., Fitzner A.

Biotechnologia

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1997

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issue 1

131-137

EN

This paper describes the present FMD epizootic situation and virus isolation and identification methods, including new molecular biology diagnostic techniques.

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Epithelial cell cultures as a model system to study the transepithelial transport

Processes involved in the repair of injured airway epithelia

Foot and mouth disease (FMD) - actual diagnostic methods