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GM-CSF regulation in eosinophils

100%

Esnault S., Malter J. S.

Archivum Immunologiae et Therapiae Experimentalis

2002

vol. 50

issue 2

121-130

Allergic asthma is characterized by a temporally and quantitatively inappropriate immunologic response. One of hallmarks of this response is the accumulation of eosinophils in the airway and lung parenchyma, which results in bronco-constriction, lung damage and ultimately fibrosis. GM-CSF plays a pivotal role in this process by modulating eosinophil function and survival. In this review, we discuss the effects and molecular regulation of GM-CSF secretion by eosinophils. Recent data demonstrate activated eosinophils release small amounts of anti-apoptotic GM-CSF by stabilizing its coding mRNA.

Immunomodulation by alpha(1)-proteinase inhibitor: lack of chemotactic effects of recombinant human alpha(1)-proteinase inhibitor from yeast on human peripheral blood granulocytes

88%

Mosheimer B., Alzner R., Wiedermann C. J.

2007

vol. 55

issue 6

399-403

Introduction: Recombinant alpha(1)-proteinase inhibitor, clinically developed for inhalative augmentation therapy in patients with alpha(1)-proteinase inhibitor deficiency or cystic fibrosis, may directly contribute to leukocyte accumulation as it may function as a chemoattractant. The migratory effects of yeast-derived human recombinant alpha(1)-proteinase inhibitor on human peripheral blood neutrophils and eosinophils were therefore tested in vitro. Materials and Methods: Human peripheral blood leukocytes were prepared from forearm venous blood and tested for migration toward various preparations of yeast-derived recombinant alpha(1)-proteinase inhibitor in modified Boyden-chamber micropore filter assays. Results: No direct effects of yeast-derived recombinant human alpha(1)-proteinase inhibitor on in vitro migration of isolated neutrophils or eosinophils were seen. Conclusions: The lack of direct chemotactic effects of recombinant human alpha(1)-proteinase inhibitor despite anti-inflammatory effects in other biological activities of leukocytes may contribute to the preserved antibacterial defense mechanisms observed in patients under experimental augmentation therapy with inhaled alpha(1)-proteinase inhibitor.

Comparison of leukocyte populations from bronchoalveolar lavage and induced sputum in evaluation of cell composition and nitric oxide production in patients with bronchial asthma

75%

Bienkowska-Haba M., Cembrzynska-Nowak M., Liebhart J., Dobek R., Liebhart E., Siemieniec I., Panaszek B., Obojski A., Malolepszy J.

Archivum Immunologiae et Therapiae Experimentalis

2002

vol. 50

issue 1

75-83

Bronchoalveolar lavage (BAL) or induced sputum (IS) techniques may provide leukocytes for the evaluation of airway inflammatory response in bronchial asthma. The aim of the present study was to compare features of leukocyte populations obtained by the two different methods regarding the cell types and their activity in patients with bronchial asthma. The nitric oxide (NO) level released from the cells was measured as a marker of their activity. Pulmonary leukocytes were obtained from the BAL and IS of 11 asthmatic patients in stable condition at the time of the study. The BAL and IS leukocyte populations varied in cell count and NO production. Macrophages were the predominant leukocyte population in BAL (Me = 83.0%, range 67.9-88.4%), whereas sputum sediments were found to consist mainly of neutrophils (Me = 55.7%, range 29.0-64.9%). The IS leukocytes released much more NO (p = 0.0022) than the BAL leukocytes. In spite of these quantitative differences, a similar pattern of NO production was observed in BAL and in IS cells. Both BAL and IS leukocyte populations produced almost the same amounts of NO before and after lipopolysaccharide stimulation (p = 0.9063, p = 0.4801, respectively). Furthermore, a slight positive correlation (RS = 0.5578, p=0.0594) was noticed between the neutrophil percentages and NO levels produced by BAL cells, whereas in IS a statistically significant correlation between the percentage of neutrophils and the levels of NO (RS = 0.6643, p = 0.0184) was observed. In conclusion, the BAL and IS leukocyte populations are different in cell type, their size and activity. Depending on the asthma severity and the type of cells needed in a study, either BAL or IS specimens may be chosen as a source of pulmonary leukocytes. The use of IS as a noninvasive technique is supposed to be potential value particularly in the study of the airway inflammatory response mediated mainly by neutrophils, i.e. during and/or after exacerbation of the disease. Based on our results, a possible contribution of neutrophils in the production of NO in the airways of asthmatic patients can be proposed apart from other cells such as macrophages.

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3 Archivum Immunologiae et Therapiae Experimentalis

1 2007

2 2002

1 Alzner R.

1 Bienkowska-Haba M.

1 Cembrzynska-Nowak M.

1 Dobek R.

1 Esnault S.

1 Liebhart E.

1 Liebhart J.

1 Malolepszy J.

1 Malter J. S.

1 Mosheimer B.

1 Obojski A.

1 Panaszek B.

1 Siemieniec I.

1 Wiedermann C. J.

Search results

GM-CSF regulation in eosinophils

Immunomodulation by alpha(1)-proteinase inhibitor: lack of chemotactic effects of recombinant human alpha(1)-proteinase inhibitor from yeast on human peripheral blood granulocytes

Comparison of leukocyte populations from bronchoalveolar lavage and induced sputum in evaluation of cell composition and nitric oxide production in patients with bronchial asthma