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1

Mouse embryonal stem calls - a tool of cloning mammals

100%
Karasiewicz J., Modlinski J. A.
Biotechnologia
|
1995
|
issue 3
84-90
EN
Mouse embryonal stem cells (ESCs) are embryo-derived totipotent cells which can be used to massively clone mice.The methods of obtaining ECSs are described and their applications and limitations in cloning are discussed.Attemps to obtain ECSs from farm animals are discussed.
2

Characterization and application of embryonic stem cells ESC in animal biotechnology

51%
Lechniak D.
Biotechnologia
|
1993
|
issue 2
55-62
EN
The micromanipulations of embryos and gametes are important tasks in animal biotechnology. These techniques can be used in animal cloning and transgenesis. For achieving these goals, embryonic stem cells are of principial interest. In vitro cultures of ESC can be derived from whole embryos or isolated inner cell masses (ICM). The attraction of ESC to animal biotechnological research is their pluripotency. ESC are able to contribute to all tissues, including germ lines. To obtain transgenic animals, ESC can be genetically transformed prior to the introduction to embryos. Present status of knowledge of ESC and their possible usefulness for genetic improvement of farm animals are discussed.
3

Mutagenesis of mouse embryonic stem (ES) cells

51%
Grundland C.
Biotechnologia
|
1999
|
issue 1
94-105
EN
Embryonic stem (ES) cells derived from preimplantation mouse embryo provide a powerful tool for genome manipulation in mammals. The two principal genetic approaches are used to modify genomes of embryonic stem cells, which may be introduced into blastocyst to produce chimeras, and these animals transmit the genetic alteration into the next generation. One approach, targeted mutagenesis, is designed to disrupt the function of specific murine genes that are known by their homology to genes of other organisms. The other approach, gene trapping by randomly insertional mutagenesis, is designed to identify novel, developmentally regulated genes in mouse embryos. In vivo screens allow for the identification and studying of genes that are expressed either within specific tissue or in spatiotemporal patterns. As an alternative to in vivo gene study, gene expression within specific cell types may be monitored in different ES cell cultures.
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