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1

Elicitation of secondary metabolites in vitro culture of Ammi visnaga

100%
Krolicka A., Lojkowska E.
Biotechnologia
|
2000
|
issue 4
112-117
EN
Ammi visnaga (Umbelliferae) are subtropical annual plants, which contain two groups of pharmaceutically important substances: furanochromones and piranocoumarins. In order to check the possibility of the production of secondary metabolites, in vitro cultures of callus and cell suspension were established. The study was concentrated on the induction of production of secondary metabolites by exposing callus and cell suspension cultures to abiotic elicitors: acetylsalicylic acid, jasmonic acid and suspension of silicon dioxide and biotic elicitors: autoclaved lysates of Enterobacter sakazaki, and scleroglucan. Thin layer chromatography of methanol extracts of cultures of A. visnaga did not indicate high induction of secondary metabolites production. Treatment of the callus cultures of A. visnaga with acetylsalicylic acid or jasmonic acid induce accumulation of furanochromone - visnagin and piranocoumarin ? samidin. Exposing the callus and cell suspension cultures to the suspension of silicon dioxide indicated an induction of accumulation of furanochromone - kelolglucoside. Further research will concentrate on quantitative determination of the level of accumulated compounds.
2

Induction of glucotropaeolin biosynthesis and myrosinase activity in hairy roots of nasturtium

75%
Wielanek M., Urbanek H.
Biotechnologia
|
2000
|
issue 4
123-127
EN
Kinetin, BAP and NAA increased glucotropaeolin content in dry weight of nasturtium hairy roots but they inhibited biomass growth. Salicylates most strongly (by about 100-200% above control) and DL-?-aminobutyric acid and methyl jasmonate to a lesser degree increased glucotropaeolin yield. They also slightly increased myrosinase activity. Trans-cinnamic acid was a much stronger inducer of myrosinase activity than glucotropaeolin biosynthesis: it strongly inhibited biomass growth. L-1-amino-2-phenylethylphosphonic acid, inhibitor of L-phenylalanine ammonia-lyase (PAL), induced glucotropaeolin production and enhanced the effect of salicylates and jasmonate on glucotropaeoplin yield but it did not affect myrosinase activity.
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