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1

Elution of glycoproteins blotted from polyacrylamide gel

100%
Pilat Z., Ficinska J., Bienkowska-Szewczyk K., Szewczyk B.
Postępy Higieny i Medycyny Doświadczalnej
|
1996
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vol. 50
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issue 5
541-544
EN
The paper describes the procedure of efficient elution of glycoproteins from Immobilon membrane after their blotting from polyacrylamide gel. The yield of elution from membrane is 30-70%.
2

Organ-specificity of esterase isoenzymes in Brassica seedlings

80%
Wilkins A., Ratajczak L., Czosnowski E.
Genetica Polonica
|
1993
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vol. 34
|
issue 2
147-152
EN
in roots epicotyls and leaves of 5-, 6-, and 7-week-old Brassica seedlings were studied. No variation in isoesterase phenotypes was found between individuals within the examined cultivar. Differences in the number and intensity of isoesterase bands between the investigated organs were observed. Distinctive isoesterase patterns were evaluated for each organ. Some esterase isoenzymes are proposed as markers of particular organs: EST 1/1 and EST 1/2 for leaves, whereas EST 2/2 and EST 2/3 for roots. Further studies be aimed at using different organo-specific isoesterase forms as markers of early stages of in vitro organogenesis in Brassica callus tissue culture.
3

Electrophoretic seed globulin patterns and species relationships in Vicia section Faba (Fabaceae)

80%
Zimniak-Przybylska Z., Przybylska J.
Journal of Applied Genetics
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1995
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vol. 36
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issue 4
299-312
EN
This work presents electrophoretic seed globulin data obtained fro 173 accessions representing nine Vicia species of the section Faba, whivh were already investigated for electrophoretic seed albumin patterns.Electrophoretic analysis of seed globulins was performed using sodium dodecyl suphate-polyacrylamide gel electrophoresis (SDS-PAGE).In the examined material totally 38 well-defined polypeptide bands, in the relative molecular mass range of 19-61 kD, were distinguished.The presence/absence of particular bands was recorded for each analysed individual.The electrophoretic data were used for hierarchical grouping of the examined taxa, based on calculated Bhattacharya distances.V bithynica proved to be the most distinctive member of Vicia section Faba.In turn, V.faba was clearly different from species of the V.narbonensis varieties and another - of the remaining species.In the latter group, V.serratifolia appeared to be rather distantly related to V.eristaliodes, V.galilaea, V.hyaeniscyamus, V.johannis and V.kalakhensis.The obtained results are compared with the corresponding seed albumin data and discussed with reference to taxonomic relatioships in Vicia section Faba.
4

Isozyme polymorphism and genetic structure of the population of Sorbus torminalis (L.) Crantz from the Bytyn Forest (Poland)

80%
Bednorz L., Myczko L., Kosinski P.
|
|
issue 3
321-324
EN
Dormant buds collected from 35 wild service trees (Sorbus torminalis) in the Bytyn Forest were tested with horizontal gel electrophoresis to assess the genetic structure of the population. Among 16 investigated isozyme loci, seven loci (ADH-A, 6PGD-A, GDH-B, ME-A, SOD-A, PGM-A, PGM-B) proved to be polymorphic, whereas the other nine loci (SDH-A, SDH-B, DIA-C, DIA-D, FLE-A, FLE-B, GOT-B, IDH-A, IDH-B) were monomorphic. The number of alleles per polymorphic locus ranged from two to three, with a mean of 2.29. The average observed and expected heterozygosities were 0.2665 and 0.3462, respectively. The combined FIS value over all polymorphic loci was 0.2179, which reflects a substantial deficit of heterozygotes. Two polymorphic loci (SOD-A, PGM-A) were identified in S. torminalis for the first time.
5

Genetic variability among beech (Fagus sylvatica L.) populations from the Sudety Mountains, in respect of peroxidase and malate dehydrogenase loci

80%
Krzakowa M., Matras J.
Journal of Applied Genetics
|
2005
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vol. 46
|
issue 3
271-277
EN
Individual trees growing in five populations of European beech (Fagus sylvatica L.) in the Sudety Mountains were investigated in respect of variability of peroxidases (2 loci) and malate dehydrogenase (1 locus). Differences between populations were illustrated by a dendrogram constructed on the basis of Hedrick's (1974) genetic distances. The mean GST coefficient (=0.0333) value demonstrated the higher level of intra-population variability, as compared to the inter-population (DST = 0.0149) variability.
6
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Postmicrosomal protein fractions from short-time-predegenerated rat sciatic nerve

80%
Gorka D., Lewin-Kowalik L.-K. J., Lewin-Kowalik J., Larysz-Brysz L.-B. M., Larysz-Brysz M., Golka G. B., Golka B., Swiech-Sabuda S.-S. E., Swiech-Sabuda E., Malecka-Tendera M.-T. E., Malecka-Tendera E.
Acta Neurobiologiae Experimentalis
|
2000
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vol. 60
|
issue 4
437-445
EN
The postmicrosomal protein fraction obtained from distal stumps of rat sciatic nerves at 0-6 days following transection were investigated by means of one- and two-dimensional electrophoresis. In all experimental groups, total amount of protein was significantly higher than in the control group. Proteins were resolved into 27 bands after SDS-PAGE. Their molecular weights ranged between 16.2 and 335.4 kDa. Eleven fractions displayed significant quantitative differences. After 2-D-electrophoresis, the pI of the proteins ranged from 4.2 to 7.4. They were resolved to 28 molecular masses from 13.5 kDa to 335.4 kDa. The greatest numbers of fractions (90-109) were observed on the 3rd, 4th, 5th and 6th day after nerve transection. Thus, during first 6 days after transection intensive changes in protein fraction content and composition take place in the distal stump of peripheral nerve. These processes seem to be most prominent on the 4th day after lesion. Results confirm our earlier in vivo findings.
7

Two-dimensional electrophoresis patterns of total proteins, esterases and acid phosphatases from pollen, seeds and leaves of three cultivars of Helianthus annuus L.

70%
Kalinowski A., Klimko M., He L., Kluza M.
Journal of Applied Genetics
|
2002
|
vol. 43
|
issue 4
437-450
EN
Total proteins, esterases and acid phosphatases from pollen, seeds and leaves of three sunflower cultivars were separated by 2-D electrophoresis. The characteristic peptides for each cultivar were identified. The seeds and pollen of the cultivar Wielkopolski contained 45 and 37 characteristic peptides, respectively, while the seeds and pollen of Coril contained 73 and 35 characteristic peptides. The cultivar Frankasol had the lowest total number of stained peptides in seeds and pollen, and the number of the characteristic peptides was only 61 and 25, respectively. Analyses of esterases and acid phosphatases from young leaves and pollen led to identification of isoenzymes characteristic of the three cultivars. Only for Frankasol no specific acid phosphatase was observed, both in leaves and in pollen.
8

Phaseolin seed variability in common bean (Phaseolus vulgaris L.) by capillary gel electrophoresis

61%
Salmanowicz B. P.
Journal of Applied Genetics
|
2001
|
vol. 42
|
issue 3
269-281
EN
Phaseolin, the major seed storage protein of Phaseolus vulgaris from forty-four wild and cultivated accessions, was studied using sodium dodecyl sulphate-capillary gel electrophoresis (SDS-CGE). In total, eleven phaseolin profiles, revealing polypeptide subunit variation in the range from 45.6 kDa to 54.4 kDa, were recorded. The number of polypeptide subunits recorded in particular profiles varied from 3 to 6; in total, eight phaseolin subunits were distinguished in the examined material. Ferguson plot analysis was used to correct non-ideal behaviour of phaseolin polypeptide subunits in capillary gel electrophoresis in the presence of SDS. The obtained results are compared to electrophoretic data received by slab polyacrylamide gel electrophoresis. The SDS-CGE method appears to provide a powerful tool for disclosure of phaseolin subunit variability.
9

Electrophoretic seed albumin patterns in the Vicia sativa L. aggregate

61%
Przybylska J., Zimniak-Przybylska Z., Krajewski P.
Journal of Applied Genetics
|
2000
|
vol. 41
|
issue 3
139-149
EN
Electrophoretic analysis of seed albumins covered 201 accessions representing six subspecies of Vicia sativa L.: nigra, amphicarpa, incisa, sativa, macrocarpa and cordata. At least five individuals of each accession were examined separately. All the taxa under study showed variation with respect to albumin banding patterns, which was due to differences between accessions and individual variation within accessions. The number of albumin bands distinguished in particular taxa varied from 13 to 19. The statistical analysis of the electrophoretic data consisted in hierarchical grouping by the UPGMA method based on EUCLIDEAN distances. In the case of subsp. nigra and subsp. sativa, accessions originating from North Africa tended to form a group showing some distinctness from the remaining accessions. As to relationships among the studied taxa, subsp. nigra and subsp. cordata were shown to be the most closely related and their affinities to other members of the V. sativa aggregate were decreasing in the following order: subsp. macrocarpa, subsp. sativa, subsp. amphicarpa, subsp. incisa. The obtained results are discussed with reference to taxonomic relationships among the members of the V. sativa aggregate.
10

Trypsin inhibitor electrophoretic patterns in Vicia faba L. and related species

61%
Zimniak-Przybylska Z., Przybylska J., Krajewski P.
Journal of Applied Genetics
|
1999
|
vol. 40
|
issue 4
283-292
EN
The studied material covered 58 accessions representing Vicia faba of section Faba, V. bithynica of section Bithynicae and seven species of section Narbonensis. Proteins of individual seeds were separated by polyacrylamide gel electrophoresis and the gels were stained for inhibitory activity against trypsin. The number of trypsin inhibitor (TI) bands recorded in particular species varied from three in V. eristalioides and V. galilaea to 15 in V. narbonensis; in total, 30 bands were distinguished in the examined material. Except for V. eristalioides, the studied species showed intraspecific variation with respect to electrophoretic TI patterns. A statistical analysis of the data, using hierarchical UPGMA grouping, resolved the studied taxa into three main clusters. V. faba subspecies/varieties formed one cluster. The second cluster consisted of V. narbonensis varieties and the rather distantly related V. eristalioides. The third cluster comprised V. bithynica as a peripheral species and the more closely associated V. galilaea, V. hyaeniscyamus, V. johannis, V. kalakhensis and V. serratifolia. The obtained results are discussed with reference to taxonomic relationships of the species under study.
11

Some examples of the use of electrophoretic protein analysis in taxonomic investigations of leguminous plants

61%
Przybylska J.
|
12

Isozymes in Aegilops kotschyi and Ae. biuncialis ? Secale cereale hybrids and Ae. kotschyi ? S. cereale amphiploids in relation to their parents

61%
Kalinowski A., Wojciechowska B., Borzyszkowska E.
Journal of Applied Genetics
|
2003
|
vol. 44
|
issue 1
35-43
EN
Seven enzymatic systems in F1 Aegilops kotschyi and Ae. biuncialis ? Secale cereale hybrids, Aegilops kotschyi ? S. cereale amphiploids and their parental species (Ae. kotschyi, Ae. biuncialis and S. cereale) were analysed by starch and polyacrylamide gel electrophoresis. Five of them (phosphoglucose isomerase, glutamic oxalacetic transaminase, esterase, acid phosphatase, and diaphorase) were polymorphic and two (malic dehydrogenase and superoxide dismutase) were monomorphic. Several isophorms of phosphoclucose isomerase, esterase, acid phosphatase, and diaphorase were detected in some hybrids and amphiploids, but absent in the parents. The role of regulators, translocations and recombination is discussed in relation to the origin of these new isophorms. Some parental isozymes were absent both in hybrids and amphiploids, probably as a result of the suppression of structural genes in new combinations of the three genomes.
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