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Cryopreservation as a method to facilitate preservation of life capacity of plant cells and tissues

100%

Mikula A.

Biotechnologia

2008

issue 2

41-57

This paper presents a review of fundamental aspects of plant cryopreservation. Liquid nitrogen has several advantages over storage of vegetatively propagated material under normal low-temperature in vitro culture and could also help in preserving genetic biodiversity. Development of efficient cryopreservation protocols based on the induction of tolerance to freezing and/or desiccation is also discussed. Cold and/or preculture acclimatization leads to ultrastructural, physiological and molecular changes in cells and they are important for improving viability after cryopreservation. The application of vitrification-based procedures and ultra-fast freezing/thawing rates could be effective and reliable for wide variety of plant species/ tissues and relatively genotype independent. Majority of papers demonstrate that the liquid nitrogen allows high viability rates and re-growth without a loss of biosynthetic capacity. Up to now, there has been no clear evidence of morphological, cytological or genetic alterations due to cryopreservation.

Methods of preservation of in vitro plant cell culture

100%

Marecik R., Kroliczak P.

issue 1

105-116

Tissue culture has been utilized for producing virus free plants for vegetative propagation and genetic engineering. In addition, altered cells lines showing higher productivity of secondary metabolities can be obtained using tissue culture. The wide use of tissue culture requires the development of new preservation techniques for in vitro culture material. One of this method is growth reduction achieved by modifying various parameters such as: temperature, culture medium, gasous environment. However, crypreservation (i. e. storage in liquid nitrogen. -196?C) is the only method available nowadays for long-term conservation. New cryopreservation techniques such as encapsulation-dehydratation, vitrification and desiccation helped expend the list of plant species that can tolerate low temperatures and are characterized by a normal rate of growth. Each step of the cryopreservation procces requires specific conditions.

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2 Biotechnologia

1 2008

1 1998

1 Kroliczak P.

1 Marecik R.

1 Mikula A.

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Cryopreservation as a method to facilitate preservation of life capacity of plant cells and tissues

Methods of preservation of in vitro plant cell culture