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Urinary incontinence in adolescent females with cystic fibrosis in Poland

100%

Korzeniewska-Eksterowicz A., Stelmach I., Stelmach W.

Open Medicine

2014

vol. 9

issue 6

778-783

Comparason of extraction methods for PCR detection of Burkholderia cepacia complex (BCC) from cystic fibrosis patients

88%

Ergunay K., Yurdakul P., Sener B., Ozcelik U., Karabulut E., Kiper N.

Open Medicine

2008

vol. 3

issue 2

157-162

Direct detection of Burkholderia cepacia complex (BCC) and its genomovars from sputum by molecular tests emerges as a method for rapid identification. In this study, four DNA extraction methods were evaluated for the identification for BCC from sputum of CF patients. Sputa from 28 CF patients were aliquoted and spiked with BCC reference strain. Boiling, phenol-chloroform, CTAB methods and a commercial spin column kit was used for DNA extraction. Total DNA yields were determined by spectrophotometry and single-round recA PCR was used for detection of BCC. No significant difference was observed in DNA yields from different extraction methods. Lower limit of detection for recA PCR was determined as 106 cfu/ml. Amplification was observed in 7/16 (43.7%) of sputa for boiling, 8/16 (50%) of sputa for CTAB and 13/16 (81.2%) of sputa for phenol-chloroform method and spin column kit in the assay sensitivity range determined in the study. Phenol-chloroform and commercial spin column kit were found to be better suited for DNA purification from sputum of CF patients for BCC identification. Diagnostic impact of single-round recA PCR directly from sputum was limited to chronically-infected patients.

Cystic fibrosis and pregnancy – literature review

63%

Krawczyk P., Sioma-Markowska U., Krówka D., Machura M., Kubaszewska S., Skrzypulec-Plinta V., Sodowska P.

Annales Academiae Medicae Silesiensis

2016

vol. 70

84-88

This paper reviews the literature on the course of pregnancy how to sustaining it as well as delivery in women with diagnosed Cystic fibrosis. Cystic fibrosis (CF) is a recessive autosomal disease occurring mostly among Caucasians. 1500 cases of the disease have been recorded in Poland and almost 70.000 people around the world suffer from it. The latest research shows that the average life span is 30 and is growing steadily due to proper diagnostics and appropriate treatment. A particular challenge for medicine is to sustain pregnancy in women with diagnosed CF. The paper collects and summarizes reccomendations for sustaining pregnancy and for childbirth in CF patients.

Mukowiscydoza jest chorobą autosomalną recesywną występującą najczęściej wśród rasy kaukaskiej. W Polsce zanotowano około 1500 przypadków choroby, na świecie na mukowiscydozę choruje blisko 70 tys. osób. Z najnowszych badań wynika, że średni okres przeżywalności szacuje się na 30 lat i ciągle wzrasta za sprawą prawidłowej diagnostyki oraz odpowiedniego leczenia. Szczególnym wyzwaniem dla medycyny staje się prowadzenie ciąży u kobiety z rozpoznaną mukowisydozą (CF – cystic fibrosis). Ciąża u kobiety chorej na CF jest ciążą wysokiego ryzyka. W pracy dokonano przeglądu danych literaturowych dotyczących przebiegu i prowadzenia ciąży oraz porodu u kobiet z rozpoznaną mukowiscydozą.

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2 Open Medicine

1 Annales Academiae Medicae Silesiensis

1 2016

1 2014

1 2008

1 Ergunay K.

1 Karabulut E.

1 Kiper N.

1 Korzeniewska-Eksterowicz A.

1 Krawczyk P.

1 Krówka D.

1 Kubaszewska S.

1 Machura M.

1 Ozcelik U.

1 Sener B.

1 Sioma-Markowska U.

1 Skrzypulec-Plinta V.

1 Sodowska P.

1 Stelmach I.

1 Stelmach W.

1 Yurdakul P.

Search results

Urinary incontinence in adolescent females with cystic fibrosis in Poland

Comparason of extraction methods for PCR detection of Burkholderia cepacia complex (BCC) from cystic fibrosis patients

Cystic fibrosis and pregnancy – literature review