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Hypothetical glycerol pathways of newly isolated strains capable of 1,3-propanediol production

100%

Leja K., Samul D., Drożdżyńska A., Myszka K., Juzwa W., Pawlicka J., Czaczyk K.

2014

vol. 61

issue 4

759-763

Study presented here demonstrates the ability of three newly isolated strains, obtained from environmental probes (manure, bottom sediment, and food waste) and identified as Clostridium bifermentans, Clostridium butyricum, and Hafnia alvei, to synthesize 1,3-propanediol (1,3-PD), organic acids (such as lactic, acetic, fumaric, succinic, and butyric acids), and ethanol from glycerol. The production of 1,3-PD as well as the glycerol pathways in C. bifermentans and H. alvei cells have not been investigated and described yet by others. Moreover, there is no data in the available literature on the products of glycerol utilization by H. alvei and there is only some incoherent data (mainly from the first half of the twentieth century) about the ability of C. bifermentans to carry out glycerol degradation. Additionally, this study presents complete hypothetical glycerol pathways and the basic fermentation kinetic parameters (such as yield and productivity) for both strains as well as for the newly isolated C. butyricum strain.

1,3-propanediol production by Escherichia coli expressing genes of dha operon from Clostridium butyricum 2CR371.5

75%

Dąbrowski S., Pietrewicz-Kubicz D., Zabłotna E., Długołęcka A.

2012

vol. 59

issue 3

357-361

1,3-propanediol is used as a monomer in the production of some polymers e.g. polytrimethylene terephthalate used in the production of carpets and textile fibers and in the thermoplastics engineering. However, the traditional chemical synthesis is expensive, generates some toxic intermediates and requires a reduction step under high hydrogen pressure. Biological production of 1,3-propanediol could be an attractive alternative to the traditional chemical methods. Moreover, crude glycerol which is a by-product of biodiesel production, can be used. We constructed a recombinant Escherichia coli strain producing 1,3-propanediol from glycerol by introducing genes of the dha operon from Clostridium butyricum 2CR371.5, a strain from our collection of environmental samples and strains. The E. coli strain produced 3.7 g of 1,3-propanediol per one litre of culture with the yield of 0.3 g per 1 g of glycerol consumed.

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2 Acta Biochimica Polonica

1 2014

1 2012

1 Czaczyk K.

1 Drożdżyńska A.

1 Dąbrowski S.

1 Długołęcka A.

1 Juzwa W.

1 Leja K.

1 Myszka K.

1 Pawlicka J.

1 Pietrewicz-Kubicz D.

1 Samul D.

1 Zabłotna E.

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Hypothetical glycerol pathways of newly isolated strains capable of 1,3-propanediol production

1,3-propanediol production by Escherichia coli expressing genes of dha operon from Clostridium butyricum 2CR371.5