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This research aims to determine the effective dosage of potassium diformate (KDF) in feed to enhance sangkuriang catfish immune performances. The research was conducted on November - January 2019, at Aquaculture Laboratory Building No. 4 Faculty of Fisheries and Marine Sciences Universitas Padjadjaran. This study used an experimental method of Completely Randomized Design (CRD), with four treatments and three replications. The treatments are the addition of potassium diformate into feed with doses A (0%), B (0.5%), C (0.7%) and D (0.9%). Observed parameters are clinical symptoms, total leukocyte count, total erythrocyte count, specific growth rate, survival rate and water quality. The results showed that 0.5% KDF feed produced the highest survival rate of 85% and the highest specific growth rate of 3.59% after 40 days. Furthermore, due to treatment, total erythrocyte count increased from 2.09×106 cells/mm3, to 2.10×106 cells/mm3 (8.1%) and total leukocyte count increased from 7.1×104 cells/mm3, to 10.7×104 cells/mm3 (42.81%). The addition of 0.5% KDF in feed is effective in improving the immune performances of sangkuriang catfish challenged by A. hydrophila, with the highest survival rate of 97%. Moreover, recovery of total leukocyte count and total erythrocyte count was faster than other treatments as of day 7th, with a value 1.97×106 sel/mm3and 12.60×104 sel/mm3. It also resulted in the lowest gross clinical signs and the fastest recovering.
EN
This study focused on determining the amylase-producing ability of the intestinal microbes in cultured fresh water fishes – Oreochromis niloticus and Clarias gariepinus. The bacterial isolates were identified on the basis of standard cultural, morphological and biochemical characteristics. The amylase production ability of the bacterial isolates was determined using starch agar. The mean viable count of the intestinal microbes ranged from 1.2 × 105 CFU/ml to 7.1 × 105 CFU/ml for tilapias (Oreochromis niloticus) and from 2.0 × 104 CFU/ml to 8.9 × 104 CFU/ml for catfishes (Clarias gariepinus). Staphylococcus and Micrococcus were predominant for both tilapias and catfishes. Out of 24 isolates, 21 were amylase producers. These included the following bacteria genera: Bacillus, Micrococcus, and Staphylococcus. These results strongly suggest that intestinal microbes play a pivotal role in the digestion of starch in cultured freshwater fishes and should be explored for industrial amylase production.
EN
This study was conducted to determine the effect of probiotic addition in commercial feed to the growth and survival rate of Sangkuriang catfish (Clarias gariepinus). This research was conducted at the Ciparanje Experimental Pond Laboratory, Faculty of Fisheries and Marine Sciences, Universitas Padjadjaran, Jatinangor, West Java. The method in used this research was Completely Randomized Design with four treatments and four replications. The treatment was the variances of probiotic addition which consisted of control (without probiotics), probiotic addition at as much as 0.5 grams / kg of feed, 1 gram / kg of feed and 1.5 grams / kg of feed. The parameters observed were specific growth rate (SGR), absolute biomass, survival rate (SR) and feed conversion ratio (FCR). Data were analyzed using Variant Analysis, at 95% confidence level, and continued with Duncan's Multiple Range Test. The results showed that the increase in the daily growth rate of Sangkuriang catfish was straight-line with the addition of probiotics. Furthermore, commercial probiotics with a dose of 1 gram / kg of feed resulted in specific growth rate of 4.22%, absolute weight of 11 grams, survival rate (SR) of 97.5% and FCR of 0.89.
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