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Ten-year prevalence of fungal peritonitis in the city of Nis, South Serbia

100%

Tasic S., Miladinovic-Tasic N., Djordjevic J., Pesic S., Avramovic M.

Open Medicine

2010

vol. 5

issue 1

49-52

Fungal peritonitis is a rare but serious complication in patients with chronic renal failure on continuous ambulatory peritoneal dialysis (CAPD). The purpose of this study was to report the prevalence of fungal peritonitis in patients on CAPD in the Clinical Center-Nis (South Serbia) in the period from 1997 until the end of 2007. Fungal species were isolated in 66 cases (4.5%) of 1471 peritoneal fluid (PF) samples that we examined. During the study period, 22 (1.5%) cases of fungal peritonitis were registered. In 19 cases, Candida isolates were identified, Candida albicans (C. albicans) being the most common species (n=15). Two cases of fungal peritonitis caused by Aspergillus fumigatus and 1 possible case caused by Cladosporium cladosporioides (Cl. cladosporioides) were also detected.

Nanosilver against fungi. Silver nanoparticles as an effective biocidal factor

88%

Pulit J., Banach M., Szczygłowska R., Bryk M.

2013

vol. 60

issue 4

795-798

The work presents a method of obtaining an aqueous raspberry extract as well as its physicochemical and analytical characteristics. The paper also contains a description of the method of preparation of nanosilver suspensions based on this extract. The raspberry extract served as a source of phenolic compounds which acted as both reducing and stabilizing agents. Suspensions of silver nanoparticles were obtained with the use of chemical reduction method. The silver ions concentration, pH value and temperature of samples incubation were independent variables. The next step of the research was to measure the antifungal activity of the received silver nanoparticles as well as to perform a mycological efficacy resistance analysis of the tested preparations in relation to different concentrations of nanostructured silver. Tests were conducted in compliance with the Eucast guidelines. The results of microbiological study of (the samples') biocidal effect against Cladosporium cladosporoides and Aspergillus niger are described. It was found that using nanosilver suspension at the concentration of 50 ppm inhibited the growth of Cladosporium cladosporoides and Aspergillus niger by 90% and 70%, respectively.

Wiązanie amfoterycyny B do upigmentowanych grzybów mikroskopowych Cladosporium cladosporioides

88%

Witoszyńska T., Kulik M., Buszman E., Trzcionka J.

Annales Academiae Medicae Silesiensis

2012

vol. 66

issue 2

34-38

BACKGROUND Dark pigmented microscopic fungi Cladosporium cladosporioides can lead to infection in immunocompromised patients. Melanin biopolymers present in the cell wall protect mycelium and are able to bind diff erent chemicals, including drugs. The aim of this work was to examine the binding capacity of antifungal antibiotic amphotericin B to crude mycelium of Cladosporium cladosporioides, melanin isolated from mycelium and to synthetic DOPA-melanin. MATERIAL AND METHODS Pigmented soil fungi Cladosporium cladosporioides were cultured in the standard liquid medium. Natural melanin was isolated from dry mycelium by acid hydrolysis. Synthetic DOPA-melanin was formed by oxidative polymerization of L-DOPA. Samples of dry mycelium and melanins were complexed with diff erent concentrations of amphotericin B using diff erent times of incubation. The amounts of drug bound to mycelium and to melanins were determined by the use of UV-VIS spectrophotometric method. RESULTS It has been demonstrated that amphotericin B forms complexes with Cladosporium cladosporioides mycelium as well as with melanin isolated from mycelium and with synthetic DOPAmelanin. The amounts of drug bound to melanins increase with increasing initial antibiotic concentration and prolongation of incubation time. CONCLUSION The ability of amphotericin B to form complexes with melanin may be one of the reasons of decreased melanin protection in relation to Cladosporium cladosporioides mycelium.

WSTĘP Ciemno upigmentowane grzyby mikroskopowe Cladosporium cladospo- rioides mogą wywoływać zakażenia, szczególnie u osób z obniżoną odpornością organizmu. Grzyby te w swojej ścianie komórkowej zawierają biopolimery melaninowe ochraniające grzybnię, zdolne do wiązania różnych związków chemicznych, w tym leków. Celem pracy była ocena zdolności wiązania antybiotyku przeciwgrzybiczego amfoterycyny B do grzybni Cladosporium cladosporioides, melaniny wyizolowanej z grzybni i do syntetycznej DOPA-melaniny. MATERIAŁ I METODY Upigmentowane grzyby glebowe Cladosporium cladosporioides hodowano w płynnej pożywce standardowej. Melaninę naturalną izolowano z grzybni metodą hydrolizy kwaśnej. Syntetyczną DOPA-melaninę uzyskano w wyniku reakcji oksydacyjnej polimeryzacji L-DOPA. Próbki melanin i grzybni kompleksowano z roztworami amfoterycyny B o różnych stężeniach, stosując różne czasy inkubacji. Do oznaczenia ilości leku związanego z grzybnią i melaninami zastosowano technikę spektrofotometrii UV-VIS. WYNIKI Wykazano, że amfoterycyna B tworzy kompleksy z grzybnią Cladospo- rium cladosporioides, z melaniną wyizolowaną z grzybni i z syntetyczną DOPA-melaniną. Ilości leku związanego z melaninami zwiększają się wraz ze wzrostem stężenia wyjściowego antybiotyku i wydłużaniem czasu inkubacji. WNIOSKI Silne oddziaływanie amfoterycyny B z melaninami może obniżać funkcje ochronne melanin w stosunku do grzybni Cladosporium cladosporioides.

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1 Acta Biochimica Polonica

1 Annales Academiae Medicae Silesiensis

1 Open Medicine

1 2013

1 2012

1 2010

1 Avramovic M.

1 Banach M.

1 Bryk M.

1 Buszman E.

1 Djordjevic J.

1 Kulik M.

1 Miladinovic-Tasic N.

1 Pesic S.

1 Pulit J.

1 Szczygłowska R.

1 Tasic S.

1 Trzcionka J.

1 Witoszyńska T.

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Ten-year prevalence of fungal peritonitis in the city of Nis, South Serbia

Nanosilver against fungi. Silver nanoparticles as an effective biocidal factor

Wiązanie amfoterycyny B do upigmentowanych grzybów mikroskopowych Cladosporium cladosporioides