Full-text resources of PSJD and other databases are now available in the new Library of Science.
Visit https://bibliotekanauki.pl
Preferences help
Polish version English version Language
enabled [disable] Abstract
Number of results

Results found: 2

Number of results on page
first rewind previous Page / 1 next fast forward last

Search results

Search:
in the keywords:  CYTOMEGALOVIRUS
help Sort By:

help Limit search:
first rewind previous Page / 1 next fast forward last
1

Immunosuppressive agents and cytomegalovirus infection

100%
Tanaka K.
Archivum Immunologiae et Therapiae Experimentalis
|
2003
|
vol. 51
|
issue 3
179-184
EN
Cytomegalovirus (CMV) infection is the major infectious complication observed after organ transplantation. As rejection episodes always occur in allograft-transplanted recipients, various kinds of immunosuppressive agnets are used to control such rejection episodes. Among the commonly used immunosuppressive agents, anti-pan T cell monoclonal antibody (OKT3) is known to increase the risk of viral infections. A new immunological techniques have recently been developed to measure CMV-specific CD4 and CD8 cells by flowcytometry. Using the techniques, the high frequencies of specific CD4 and CD8 T cells have been shown to be required to survey the CMV (re)activation in the persistent/latent phase of CMV infection. An excessive T cell depletion by OKT would deplete such surveying T cells, thus resulting in the occurrence of CMV-associated diseases.
2

Comparison of two methods used for monitoring low-copy cytomegalovirus infection in a patient with chronic myeloid leukemia after unrelated umbilical cord blood transplantation

100%
Dzieciatkowski T., Przybylski M., Tomaszewska A., Rokicka M., Luczak M.
|
2007
|
vol. 55
|
issue 3
199-203
EN
Introduction: Detection of human cytomegalovirus (CMV, HHV-5) DNA in clinical specimens is considered a cornerstone in the diagnosis of HHV-5 disease. The present study compared two quantitative methods used for diagnosing cytomegalovirus infection in a 21-year-old woman with chronic myeloid leukemia after an unrelated umbilical cord blood transplantation. Materials and Methods: Blood samples were tested for the presence of HHV-5 DNA using the LightCycler PCR, the quantitative Eclipse? CMV DNA Detection Kit, and a qualitative in-house PCR assay using primers that amplify part of the HHV-5 MIE gene. Results: Results from samples containing a low cytomegalovirus load were more accurate with the LightCycler test than those obtained with the Eclipse? test, which underestimated the viral load of samples containing low DNA copy numbers. Conclusions: These findings underline the value of novel PCR methods used in current therapeutic procedures and in monitoring antiviral therapy with nucleoside analogs. The high level of sensitivity, specificity, accuracy, and rapidity provided by the LightCycler instrument are favorable for the use of this system in the detection of HHV-5 DNA in clinical specimens.
first rewind previous Page / 1 next fast forward last
JavaScript is turned off in your web browser. Turn it on to take full advantage of this site, then refresh the page.