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Cryopreservation of plant cell culture

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van Iren F., Schrijnemakers E., Reinhoud P.
Biotechnologia
|
1996
|
issue 1
60-68
EN
Some relatively simple and cheap procedures for long term preservation in liquid nitrogen have been worked out.These are based both on two-step freezing (in which the specimen is progressively dehydrated when ice crystals grow in the medium during slow cooling) and on vitrification (absence of ice crystals due to rapid freezing in highly concentrated vitrifying solutions).For recalcitrant strains alternative storage methods have been studied.
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