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1

Differences in the inheritance stability of kanamycin resistance between transgenic cucumbers (Cucumis sativus L.) containing two constructs

100%
Yin Z., Szwacka M., Malinowski R., Malepszy S.
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issue 3
307-314
EN
A phenotypic segregation of kanamycin resistance conferred by nosnptII was investigated in two groups of transgenic cucumber lines one containing the PR-2duidA-nosnptII construct and the other with the thaumatinII-nosnptII construct to the third and fifth generation, respectively. Expression of the nptII gene was more stable and predictable in the progeny of the PR-2d lines than that of thaumatin lines. In T2 and T3 generations representing seven PR-2d primary transformants, 78% of the hemizygous progenies exhibited a segregation ratio consistent with Mendelian inheritance; non-Mendelian ratio occurred in the remaining 22%. Mendelian segregation was observed in 46% of the hemizygous progenies derived from 11 thaumatin primary transformants. The segregation ratio for two and three independent loci appeared in single PR-2d and thaumatin lines, respectively.
2

Transformation of cucumber Cucumis sativus L.

100%
Szwacka M., Burza W., Palucha A., Malepszy S.
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EN
Different transformation methods were investigated. However a lot of fertile plants were obtained only in the case of A. tumefaciens and thaumatin with kanamycin as a selective marker. Small differences in the plant morphology and development were observed in T0 generation. Most of the plants showed the presence of the transgene but the level of mRNA was different.
3

Genetic background of host-pathogen interaction between Cucumis sativus L. and Pseudomonas syringae pv. lachrymans

88%
Olczak-Woltman H., Schollenberger M., Niemirowicz-Szczytt K.
Journal of Applied Genetics
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2009
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vol. 50
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issue 1
1-7
EN
The interplay of plant resistance mechanisms and bacterial pathogenicity is very complex. This applies also to the interaction that takes place between the pathogen Pseudomonas syringae pv. lachrymans (Smith et Bryan) and the cucumber (Cucumis sativus L.) as its host plant. Research on P. syringae pv. lachrymans has led to the discovery of specific factors produced during pathogenesis, i.e. toxins or enzymes. Similarly, studies on cucumber have identified the specific types of plant resistance expressed, namely Systemic Acquired Resistance (SAR) or Induced Systemic Resistance (ISR). This paper presents a summary of the current state of knowledge about this particular host-pathogen interaction, with reference to general information about interactions of P. syringae pathovars with host plants.
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