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Cryopreservation as a method to facilitate preservation of life capacity of plant cells and tissues

100%

Mikula A.

Biotechnologia

2008

issue 2

41-57

This paper presents a review of fundamental aspects of plant cryopreservation. Liquid nitrogen has several advantages over storage of vegetatively propagated material under normal low-temperature in vitro culture and could also help in preserving genetic biodiversity. Development of efficient cryopreservation protocols based on the induction of tolerance to freezing and/or desiccation is also discussed. Cold and/or preculture acclimatization leads to ultrastructural, physiological and molecular changes in cells and they are important for improving viability after cryopreservation. The application of vitrification-based procedures and ultra-fast freezing/thawing rates could be effective and reliable for wide variety of plant species/ tissues and relatively genotype independent. Majority of papers demonstrate that the liquid nitrogen allows high viability rates and re-growth without a loss of biosynthetic capacity. Up to now, there has been no clear evidence of morphological, cytological or genetic alterations due to cryopreservation.

Germin genes expression induced by stress factors in transgenic tobacco plants (Nicotiana tabacum var. Xanthi)

72%

Nowakowska J.

Biotechnologia

2001

issue 2

152-164

Wheat germin, a glycoprotein mainly localized in the cell wall of monocots, dicots and pine (Pinus caribaea Morelet), is a specific marker of the onset of growth in germinating seeds. There are two genes gf-2.8 and gf-3.8 of germin, isolated from wheat (Triticum aestivum). Because of their structure and biochemistry, both of the germin's isoforms have been now assimilated to the oxalate-oxidase enzyme (E.C. 1.2.3.4). Several constructs of the gf-2.8 and gf-3.8 genes promoters deletion were fused with uidA gene and tested for the GUS activity in the transgenic tobacco plants. The aim of this work was to evaluate whether some environmental factors induce the activity of germin. Hormonal treatment (auxins 2,4-D, NAA, gibberellin GA3), salt treatment, salicylic acid, heavy metals (Mn, Fe, Co, Ni, Cu, Zn, Cd, Hg, As) and A1 induced high GUS activity in root apex, cotyledons and hypocotyls in transgenic seedlings and in leaves of the tobacco plants transformed with the entire and one partially deleted construct of the gf-2.8 gene. Neither cold nor heat shock, enhanced significant GUS activity. The gf-3.8 - GUS constructs of germin did not give a positive response to any factors mentioned above. Thus, only the gf-2.8 protein seems to be regulated by some factors. The gf-2.8 oxalate-oxidase activity could be then involved in general stress-induced signalling in higher plants

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2 Biotechnologia

1 2008

1 2001

1 Mikula A.

1 Nowakowska J.

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Cryopreservation as a method to facilitate preservation of life capacity of plant cells and tissues

Germin genes expression induced by stress factors in transgenic tobacco plants (Nicotiana tabacum var. Xanthi)