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Resistance of transgenic tobacco lines to Potato Virus Y

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Czubacka A., Doroszewska T.
Biotechnologia
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2010
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issue 2
72-82
EN
Potato virus Y (PVY) causes a disease which reduces the quality of raw material as well as the yield. This study on resistance included tobacco transgenic lines belonging to four cultivars modified with three genetic constructions, containing Lettuce mosaic virus coat protein gene (LMV CP) and PVY replicase gene used in sense (ROKY1) and antisense (ROKY2) orientation. The plants were inoculated with PVY isolates under greenhouse conditions and tested by DAS-ELISA. The highest effectiveness of antiviral protection was noticed in case of the lines with transgene LMV CP, lower for ROKY2, and the lowest for ROKY1. In following generations, the percentage increase of a number of plants resistant to PVY was observed within the lines containing transgene LMV CP. Cultivar MN 944 showed to be the best genetic background for that transgene, while cultivar AC Gayed for transgene ROKY.
2

Transgenic lines of rape resistant to infection of turnip mosaic virus (tum

100%
Lehmann P., Kozubek E., Wojciechowski A.
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issue 1
129-139
EN
Transgenic Brassica napus var. oleifera cv. Westar plants that express the coat protein gene of the turnip mosaic virus (TuMV) were generated. The transgenic plant lines that contained one, two and at least three transgenes inserts in both homozygous and hemizygous conditions were produced and examined. Twelve different transgenic plant lines (in T3 generation) were analysed for resistance to TuMV. Three different responses were observed when the transgenic plant lines were inoculated with TuMV; 1) some were highly reistant, 2) some were medium resistant and, 3) some were susceptible to TuMV infection. We did not find any correlation between the number of copies of the transgene and virus resistance. The most promissing transgenic line was used to breed a few winter cultivars of rape to induce the pathogen-mediated virus resistance in these cultivars.
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