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Importance of Urban Factor and Selected Socio-Economic Variables in the Differentiation of Coordination Motor Abilities Level (CMA)

100%

Jaworski J., Madejski E.

Polish Journal of Sport and Tourism

2011

vol. 18

issue 3

197-200

Introduction. The aim of this study was to investigate the effects of the urban factor and socio-economic status on selected coordination motor abilities of non-practicing physical education female students. Material and methods. The research covered 83 female students aged 20.6±0.8 SD years. Data on place of residence and socio-economic status of respondents were collected through a survey. The study of coordination motor abilities was carried out by a special computer application using for this purpose a "tablet" with touch screen. Assessment of the size, scope and direction of differentiation between the groups was made on the basis of standardized inter-group differences. Results. The inter-group differences were presented in the surveyed female students' coordination motor abilities, depending on the urban factor and socio-economic status. The gradient of these changes was as follows: the city over 25 thousand population > city of 25 thousand population > small town. The scale of differentiation was dependent on the type of tested abilities and the environmental factor. Conclusion. Gradient of observed changes could be caused by, e.g. more environmental stimuli stimulating the nervous system in the earlier periods of development of the female students from larger urban clusters and families with higher socio-economic status.

Sequential fluorescent staining with CMA and DAPI for somatic chromosome identification in cucumber (Cucumis sativus L.)

100%

Plader W., Hoshi Y., Malepszy S.

Journal of Applied Genetics

1998

vol. 39

issue 3

249-258

Each of the seven chromosomes in cucumber (Cucumis sativus L.) was identified using sequential staining with Chromomycin A3 (CMA) and 4-6-diamidino-2-phenylindole (DAPI) as DNA base-specific fluorescent dyes. The present method using enzymatic digestion produced a high level of well-spread early-metaphase chromosome complements. After CMA and DAPI staining, reproducible fluorescence bands were obtained in mitotic prometaphase chromosomes. The CMA staining method made it possible to characterize whole chromosomes from prometaphase to mid-metaphase. Chromosome 1 had the largest and widest CMA-positive (CMA+) band from the proximal region to the interstitial region on the long arm in prometaphase. A large gap separating of the short arm from the long arm was always observed in chromosome 2 during prometaphase. The banding pattern of the short arm was similar to that of the long arm in chromosome 2. Chromosomes 1 and 2 in early metaphase had sharp and large CMA-positive and DAPI-negative (CMA+DAPI-) bands at the pericentromeric regions. In early metaphase, chromosome 3 was characterized by having a narrow CMA+DAPI- band on the pericentromeric region of the short arm. Chromosomes 4 and 5 showed similar chromosome length and had a large CMA+ band at the distal region of the long arm. Chromosome 4 did not show any clear band in the short arm, while chromosome 5 showed a telomeric CMA+ band at the short arm and a clear CMA+DAPI- band at the pericentromeric region. Chromosome 6 had a CMA+ band at the distal region and a weak CMA+ band at the proximal region in each of the arms. Chromosome 7 had an evident CMA+ band in the long arm and a CMA+DAPI- band in the pericentromeric region.

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1 Journal of Applied Genetics

1 Polish Journal of Sport and Tourism

1 2011

1 1998

1 Hoshi Y.

1 Jaworski J.

1 Madejski E.

1 Malepszy S.

1 Plader W.

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Importance of Urban Factor and Selected Socio-Economic Variables in the Differentiation of Coordination Motor Abilities Level (CMA)

Sequential fluorescent staining with CMA and DAPI for somatic chromosome identification in cucumber (Cucumis sativus L.)