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Why is mutation breeding still attractive for breeding of chrysanthemum?

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Miller N.

Biotechnologia

2005

issue 2

196-205

Many cultivars of chrysanthemum as well as other ornamental crops on Polish market are of foreign origin. Such situation is not to the advantage of Polish producers because of high royalties. There is a necessity of providing new Polish cultivars of ornamental crops, including chrysanthemum. Traditional breeding methods such as cross-breeding are not expensive but take much time and cannot be always applied for ornamental crops, which are usually heterozygous, poliploids and are vegetatively propagated. On the other hand, there are modern techniques including genetic transformation, but they are often out of reach for Polish breeders due to very high costs. Mutation breeding is a relatively inexpensive and easy method of obtaining new cultivars. Twelve new cultivars of chrysanthemums have been obtained as a result of mutation breeding research, carried out in the Departament of Ornamental Crops and Vegetables at University of Technology and Agriculture in Bydgoszcz. Induced mutations most often led to altered inflorescensce colour and shape, growth habit and other traits. Both induced mutations and regeneration in vitro using adventitious buds technique apear to be useful tools in breeding of chrysanthemum.

Topophysis in adventitious shoots regeneration in vitro in chrysanthemum

100%

Zalewska M., Miler N.

Biotechnologia

2010

issue 2

89-95

Success of breeding programmes using adventitious shoots technique often depends on the regeneration efficiency. The aim of this study was to investigate the influence of topophysical position of explants on the efficiency of adventitious shoots regeneratio . Uniform, single shoots of chrysanthemum 'Satinbleu' propagated in vitro were divided equally into three topophysical zones: distal, central and proximal. Two leaves and two internodes were isolated from each zone. The explants were cultured for 12 weeks on MS medium supplemented with 0.6 mg l-1 BAP and 2.0 mg l-1 IAA. Due to the highest adventitious shoots regeneration capability and the earliest formation of the shoots, the most suitable explants for breeding programmes for chrysanthemum are internodes excised from distal part of in vitro plantlet.

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2 Biotechnologia

1 2010

1 2005

1 Miler N.

1 Miller N.

1 Zalewska M.

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Why is mutation breeding still attractive for breeding of chrysanthemum?

Topophysis in adventitious shoots regeneration in vitro in chrysanthemum