Karyotypes of 8 species of Psyllina (Strophingia ericae, Craspedolepta flavipennis, C. malachitica, Rhinocola aceris, Psyllopsis fraxinicola, Cyamophila cinnamomea, Psylla alni, and Psylla eucallypti) were described for the first time. The chromosome number for a further 14 species is summarized on the basis of the literature data.
The results of karyological studies of Curculionidae published since 1978 are summarized. Chromosome complements for 189 species are presented. Diploid chromosome number 2n=22, and meioformula n%=10+Xyp is the most characteristic chromosome number of weevils occuring in more than one-third of species examined karyologically. In Curculionidae there is a clear tendency towards symmetrization of karyotypes.
Karyotype variation in Psylloidea (Hemiptera) was analysed. Chromosome numbers and type of chromosome sex determination of 153 species belonging to 51 genera and five out of six families (except tropical Phacopteronidae) are listed. Some important cytological characteristics, such as chromosome type, structure of karyotypes, sex determination type, pattern of male meiosis, type of chromosome rearrangements, and B chromosome occurrence, as well as the C-heterochromatin and NOR localization, are discussed. In addition, a comparison of chromosome systems between Psylloidea and other superfamilies of Sternorrhyncha (Aphidoidea, Coccoidea, and Aleyrodoidea) was undertaken.
The present study aimed to identify morphological traits whose performance depends on plant ploidy in Chamomilla recutita. Flowerhead diameter, 100-flowerhead weight, 1000-seed weight, pollen grain diameter, stoma length, and number of chloroplasts per guard cell, were examined in 5 tetraploid and 5 diploid strains. Out of these traits, stoma length, number of chloroplasts per guard cell, 100-flowerhead weight, and 1000-seed weight, proved to be significantly higher in tetraploid than in diploid strains, and can be used for indirect identification of tetraploid and diploid genotypes in various developmental stages of this species.
Karyotypic details were studied in males of eight beetle species from four families, viz. Carabidae: Elaphrus cupreus Duft. (2n=33, n=16+X), Elaphrus aureus Mull. (2n=31, n=15+X), Elaphrus ullrichii Redt. (2n=31, n=15+X); Scarabaeidae: Oxythyrea funesta (Poda) (2n=19, n=9+X); Cerambycidae: Agapanthia violacea (F.) (2n=20, n=9+Xyp), and Chrysomelidae: Oulema gallaeciana (Heyd.) (2n=16, n=7+Xyp), Colaphus sophiae (Schall.) (2n=27, n=13+X), Phaedon cochleariae (F.) (2n=34,n=16+Xyp). The chromosome number and sex determining system of four species are described for the first time. Evolutionary trends in karyotypes of the studied beetle groups are briefly discussed.
An analysis was made of the C-banded karyotype of Paederius rubrothoracicus carpathicola Scheerp. The result indicates that the chromosome number is 2n=36 and n%=17+Xyp. The examined karyotype shows a paracentromeric position of constitutive heterochromatin in all autosomes. The y chromosome is wholly euchromatic.
Cytogenetic investigations were carried out on seven species of bisexual weevils from five subfamilies. The following numbers of chromosomes were found in individual species: 2n=22, n_=10+Xyp in Ptochus porcellus Boh. (Otiorhynchinae); 2n=40, n_=19+Xyp in Larinodontes turbinatus Gyll. (Cleoninae); 2n=26, n_=12+Xyp in Curculio pellitus (Boh.) (Curculioninae); 2n=30, n_=14+Xyp in Acalles camelus (F.), Acalles echinatus (Germ.), Acalles hypocrita Boh. (Cryptorhynchinae), and 2n=40, n_=19+Xyp in Cionus hortulanus (Foucr.) (Mecininae). The heterochromosomes of all the examined species form, in the first meiotic metaphase, a parachute bivalent. The chromosome number and sex determining system of six species and two genera are described for the first time.
Karyological investigations were carried out on eight species of bisexual weevil belonging to five subfamilies, viz. - Otiorhynchinae, Brachyderinae, Cleoninae, Hylobiinae, Ceutorhynchinae.
An analysis was made of the C-banded karyotype of Strophosoma capitatum (Deg.). The results indicate that the chromosome number is 2n=22 and n%=10+Xyp. The examined karyotype shows a pericentromeric position of constitutive heterochromatin in all autosomes. The shorter arm of the X chromosome is heterochromatic while the y chromosome is wholly euchromatic. Successive stages of spermatogenesis were analysed.
Karyological studies were carried out on seven Palaearctic weevils. The following chromosome numbers were found in individual species, i.e. Otiorhynchus niger (F.), Phyllobius viridearis (Laich.), Phyllobius scutellaris Redt., Phyllobius calcaratus (F.), Polydrusus cervinus (L.), and Brachyderes incanus (L.) 2n=22, n%=10+Xyp, in Lixus elegantulus (Boh.) 2n=22, n%=21+Xyp. The heterochromosomes of all the examined species form, in the first meiotic metaphase, a typical parachute bivalent.
Karyological studies were carried out on six bisexual weevil species from five subfamilies, viz. ? Otiorhynchinae, Cleoninae, Curculioninae, Pissodinae, and Hylobiinae. The following chromosome numbers were found in individual species: in Peritelus leucogrammus Germ., Phyllobius cloropus (L.), and Hypera viciae (Gyll.) 2n = 22, n_ = 10+Xyp, in Curculio venosus Grav. 2n= 26, n_ = 12+Xyp, in Magdalis armigera (Foucr.) 2n = 36, n_ = 17+Xyp, in Lixus filiformis (F.) 2n = 44, n_ = 21+Xyp. The heterochromosomes of all the examined species form, in the first meiotic metaphase, a parachute bivalent. The mitoses were observed and analysed in three species. The karyotypes of Peritelus leucogrammus Germ., Phyllobius cloropus (L.), and Curculio venosus Grav. are constituted by metacentric and submetacentric chromosomes.
Chromosome numbers and C-banding patterns of sixteen weevil species are presented. The obtained results confirm the existence of two groups of species with either a small or large amount of heterochromatin in the karyotype. The first group comprises twelve species (Apionidae: Oxystoma cerdo, Eutrichapion melancholicum, Ceratapion penetrans, Ceratapion austriacum, Squamapion flavimanum, Rhopalapion longirostre; Nanophyidae: Nanophyes marmoratus; Curculionidae: Centricnemus (=Peritelus) leucogrammus, Sitona humeralis, Sitona lineatus, Sitona macularis, Sitona suturalis). In weevils with a small amount of heterochromatin, tiny grains on the nucleus during interphase are visible, afterwards appearing as dark dots during mitotic and meiotic prophase. The second group comprises four species from the curculionid subfamily Cryptorhynchinae (Acalles camelus, Acalles commutatus, Acalles echinatus, Ruteria hypocrita) which possess much larger heteropycnotic chromosome parts visible during all nuclear divisions. The species examined have pericentromeric C-bands on autosomes and on the X chromosome.
Karyological details were studied in males of seven weevil species. The following number of chromosomes were found in individual species: 2n=32, n_=15+Xyp in Gymnetron tetrum (F.) and Gymnetron smreczynski Fremuth, 2n=44, n_=21+Xyp in Cionus tuberculosus (Scop.), 2n=38, n_=18+Xyp in Cionus hortulanus (Geoffr.), Cionus ganglbaueri Wingelm. and Cionus nigritarsis Reitt., 2n=42, n_=20+Xyp in Cionus olivieri Rosensch. In the first meiotic metaphase the heterochromosomes of all the examined species formed a typical parachute bivalent. The chromosome number and sex determining system were described for the first time.
The C-banding pattern of Bembidion geniculatum, Silpha atrata, Prosternon tesselatum, and Epicometis hirta are presented. All analysed species have pracentromeric C-bands on autosomes and chromosome X but the widest ones are visible in the karyotype of B. geniculatum. In S. atrata, P. tesselatum, and E. hirta sex chromosome y is heterochromatic, only B. geniculatum having the Y chromosome wholly euchromatin. The results indicate that on the chromosomes of the investigated species do not have a terminal and an intercalar segments of heterochromatin.
Karyotypic details were studied in males of six beetle species from three families, viz. Tenebrionidae: Dailognatha pumila Bdy. (2n=20, n male= 9+Xyp), Pachyscelis musiva Menetr. (2n=18, n male= 8+Xyp), Pimelia capito Kryn. (2n = 18, n male= 8+Xyp); Cerambycidae: Agapanthia walteri Reitt. (2n=20, n male = 9+Xyp), Agapanthia korostelevi Danilevsky (2n=20, n male = 9 + Xyp); Curculionidae: Phyllobius caucasicus Stierl. (2n = 22, n male=10+Xyp). The chromosome number and sex determining system of all beetle species are described for the first time. Evolutionary trends in karyotypes of the studied beetle groups are briefly discussed.
C-banded karyotypes were studied in the males of seven ladybird species from the subfamily Coccinellinae, viz. Adonia variegata (Goeze), Tythaspis sedecimpunctata (L.), Coccinella septempunctata (L.), Calvia decemguttata (L.), Calvia quatuordecimguttata (L.), Propylea quatuordecimpunctata (L.), Phyllobora vigintiduopunctata (L.). All the species, with the exception of Tythaspis sedecimpunctata (L.), possess 2n=20, n male=9+Xyp. Tythaspis sedecimpunctata (L.), however, has 2n=24, n male=11+Xy. The examined karyotypes show only a paracentromeric position of constitutive heterochromatin in all autosomes and the sex chromosome X, while the y heterochromosome is dot-like and wholly euchromatic. Successive stages of spermiogenesis were analysed.
Mitotic and meiotic chromosomes were studied in nine beetle species from three families, viz. Bruchidae: Bruchus pisorum L.) (2n=34, no%=16+Xy); Apionidae: Legaricapion pisi (F.) (2n=22, n%=10+Xyp); Curculionidae: Otiorhynchus opulentus Germ., Polydrusus marginatus Steph., Polydrusus viridicinctus Gyll., Liophloeus lentus Germ., Liophloeus gibbus Boh. (2n=22, n%=10+Xyp), Larinodontes obtusus Gyll. (2n=40, n%=19+Xyp), Zacladus geranii (Payk.) (2n=28, n%=13+Xyp). In the first meiotic metaphase the heterochromosomes of eight examined species formed a typical parachute bivalent. The chromosome number and sex determining system of seven species were described for the first time.
Karyological studies were carried out on three Armenian weevil species from the subfamily Cleoninae. The following chromosome numbers were found in individual species: 2n = 38, n% = 18+Xyp in Menecleonus anxius (Gyllenhal, 1824), 2n=40, n% = 19+Xyp in Conorhynchus nigrivittis (Pallas, 1781) and 2n = 44, n% =21+Xyp in Lixus iridis Olivier, 1807. The heterochromosomes of all the examined species form, in the first meiotic metaphase, a typical parachute bivalent.
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