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Constitutional chromosome instability in families with patients affected by sporadic non-hereditary retinoblastoma

100%

Zajaczek S., Gorski B., Krzystolik Z., Lubinski J.

Journal of Applied Genetics

1999

vol. 40

issue 4

343-353

It has been suggested that chromosome instability is a constitutional factor which may be characteristic of families with an increased risk for the development of malignancies. In this study spontaneous chromosome aberrations, sister chromatid exchanges (SCEs) and chromosome sensitivity to bleomycin were analysed in cultured lymphocytes from 13 families with sporadic non-hereditary unilateral retinoblastoma patients. The significantly increased chromosome instability was detected only by the bleomycin test. Higher mean values of breaks/cell were found in all groups of relatives: patients, parents and siblings.

Molecular cytogenetic evaluation of chromosome instability in Triticum aestivum?Secale cereale disomic addition lines

70%

Szakacs E., Molnar-Lang M.

vol. 51

issue 2

149-152

The genetic stability of wheat/rye ('Chinese Spring'/'Imperial') disomic addition lines was checked using the Feulgen method and fluorescent in situ hybridization (FISH). Feulgen staining detected varying proportions of disomic, monosomic, and telosomic plants among the progenies of the disomic addition lines. The greatest stability was observed for the 7R addition line, while the most unstable lines were those with 2R and 4R additions. Chromosome rearrangements were also detected using FISH. Based on the specific hybridization patterns of repetitive DNA probes pSc119.2 and (AAC)5, as well as ribosomal DNA probes (5S and 45S), isochromosomes were identified in the progenies of 1R and 4R addition lines. The results draw attention to the importance of continuous cytological checks on basic genetic materials by using FISH, because this method reveals chromosome rearrangements that could not be detected either with the conventional Feulgen staining technique or with molecular markers.

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2 Journal of Applied Genetics

1 2010

1 1999

1 Gorski B.

1 Krzystolik Z.

1 Lubinski J.

1 Molnar-Lang M.

1 Szakacs E.

1 Zajaczek S.

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Constitutional chromosome instability in families with patients affected by sporadic non-hereditary retinoblastoma

Molecular cytogenetic evaluation of chromosome instability in Triticum aestivum?Secale cereale disomic addition lines