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1

Karyotype evolution of the established RES (Ren Embryonis Suis) cell line

100%
Dziekanowska D., Scheller S., Rokicka A., Legowik E.
Journal of Applied Genetics
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1995
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vol. 36
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issue 2
177-185
EN
The present paper refers to the previously reported cytogenetic studies of a cell line derived from an embryonic pig kidney and deals with chromosome alternations suddenly and spontanously occuring in this line as structural rearrangements and polyploidy.Successive stages of karyotype evolution, domination of abnormal karyotype and this population dynamics are described.
2

Comparative in vitro study on the adhesion of probiotic and pathogenic bacteria to different human intestinal cell lines

100%
Lewandowska M., Olejnik A., Neumann M., Krepulec A., Piotrowska J., Tresiak A., Grajek W.
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EN
The ability of four strains of Lactobacillus sp. two strains of Bifidobacterium sp. and one strain of Listeria mnocytogenes to adhere to human intestinal cell lines Caco-2, HT-29 and Int 407 was examined. Well-developed monolayers of intestinal cells were obtained when initial concentration of Caco-2 cells was 1 x 104/cm2, HT-29 cells 4.2 x 104/cm2, and Int 407 cells 2 x 104/cm2. The appropriate fetal bovine serum additions for Caco-2, HT-29 and Int 407 were 20%, 10% and 10%, respectively. The reduction of serum addition decreased intestinal cell density and prolonged monolayer development. The highest cell densities in epithelial monolayer were obtained in the Int 407 cell cultures. The yield of bacterial adhesion was strain ? dependent. Significant differences were also observed in bacteria adhesion to individual intestinal cell lines. The best adhesion ability to Caco-2 exhibited Lactobacillus rhamnosus GG and Bifidobacterium bifidum. The highest adhesion to HT-29 line demonstrated B. bifidum and Lactobacillus acidophilus LC1. The adhesion of bacteria to Int 407 was much lower. Significant effect on bacteria adhesion has their cell density being in contact with intestinal monolayer. The adherence of Listeria monocytogenes to Caco-2 and HT-29 was very low in the range of 0.2% and 6.0%, respectively.
3

Beauvericin cytotoxicity to the invertebrate cell line SF-9

75%
Calo L., Fornelli F., Nenna S., Tursi A., Caiaffa M. F., Macchia L.
Journal of Applied Genetics
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2003
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vol. 44
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issue 4
515-520
EN
The cyclic hexadepsipeptide beauvericin, initially known as a secondary metabolite produced by the entomopathogenic fungus Beauveria bassiana and toxic to Artemia salina larvae, has been more recently recognized as an important mycotoxin synthesized by a number of Fusarium strains, which parasite maize, wheat and rice. Therefore, this mycotoxin may enter the food chain, causing yet unknown effects to the health of both domestic animals and humans. The cytotoxic effects of beauvericin on mammalian cells have been studied. We investigated the cytotoxicity of this compound in an in vitro invertebrate model, viz. the insect cell line SF-9 (immortalized pupal ovarian cells of the lepidopter Spodoptera frugiperda). Cultures of SF-9 cells in the stationary phase were exposed to beauvericin at concentrations ranging from 100 nM to 300 M, for different periods of time (from 30? to 120 h). The effects on cell viability were assessed by the trypan blue exclusion method. After 4 h of incubation no significant decrease in cell viability was recorded in SF-9 cell cultures exposed to low concentrations of beauvericin, i.e. 100 nM and 300 nM. However, a slight decrease in viability (3.9%) was seen already in cells exposed to the mycotoxin at the 1 M concentration. This effect became gradually more evident at higher concentrations ( 28% at 30 M, 50% at 100 M, 68% at 300 M). An even more pronounced reduction in cell viability was observed after a 24 h exposure. Under these conditions, 1 M beauvericin caused an approx. 10% decrease in the number of viable cells, which became more significant at higher concentrations 23% at 3 M, 47% at 10 M, 65% at 30 M, 90% at 100 M, 99% at 300 M). Therefore, the 50% cytotoxic concentrations (CC50) at 4 h and 24 h could be estimated as 85 M and 10 M, respectively. In time-course experiments, no effect of beauvericin (30 M) on cell viability could be seen after exposure for periods of time as long as 30?, 1 h and 2 h, respectively. In contrast, when SF-9 cells were exposed to the mycotoxin for longer periods of time, from 8 h to 120 h, we recorded a strong cytotoxic effect already in the low micromolar concentration range. Thus, the CC50 after both 72 h and 120 h exposure times was assessed as 2.5 M. Higher concentrations caused a virtually 100% cell death. The data collected suggest that beauvericin exerts a substantial dose- and time-dependent cytotoxic effect on invertebrate cells, comparable to the effects described in mammalian cells.
4

Novel Ru(III), Rh(III), Pd(II) and Pt(II) complexes with ligands incorporating azole and pyrimidine rings. I. Antiproliferative activity in vitro

75%
Wisniewski M. Z., Wietrzyk J., Opolski A.
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vol. 48
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issue 1
51-55
EN
A number of co-ordination compounds of Ru(III), Rh(III), Pd(II) and Pt(II) with ligands incorporating azole and pyrimidine rings has been synthesized. The in vitro cell proliferation-inhibitory activity of these compounds was examined against human cancer cell lines: A 549 (lung carcinoma), LS-180 (colon cancer) and MCF-7 (breast cancer), using SRB technique. Six out of 13 compounds studied revealed cytotoxic activity in vitro. Inhibitory dose 50% (ID50) was lower than 4 g/ml, which is an activity criterion accepted in conventional in vitro cytotoxic screening tests. Two compounds revealed weak cytotoxic activity with ID50 higher than 4 g/ml and five compounds were inactive.
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