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Screening of antibiotic-producing bacteria isolated from soil gotten within gaba, Bwari FCT, Abuja

100%
Azode V. U., Anyaegbunam B. C.
World News of Natural Sciences
|
2023
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vol. 51
61-69
EN
This investigation seeks to determine the antibiotic-producing potential of bacteria in inhibiting the growth of other organisms like Staphylococcus aureus and Escherichia coli. The bacterial isolates were identified using standard cultural, morphological, and biochemical characterization. These were further screened for their antibiotic-producing potential using the Mueller Hinton agar (MHA) media. The identified bacterial isolates had a mean viable count ranging between 1.38×106 / 1,380,000 cfu/ml and 1.52×106 / 1,520,000 cfu/ml. Micrococcus roseus, Brevibacterium species, Bacillus subtilis, Bacillus anthracis, and Bacillus cereus were the predominant bacterial isolates. Nonetheless, the antibiotic test revealed that Bacillus subtilis and Bacillus anthracis showed antimicrobial activity against S. aureus, whereas Bacillus anthracis, Bacillus cereus, and Bacillus subtilis showed zones of inhibition against E. coli. This study shows that Bacillus species have the potential to produce antibiotics and can be used to control microbial growth in the future.
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Bacteriological Evaluation of Hydrocarbon Polluted Soil in Obitti Oil Field, Ohaji-Egbema, Imo State, Nigeria

75%
Maduwuba M. C., Akwarandu J. O.
World News of Natural Sciences
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2023
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vol. 49
137-146
EN
The bacteriological evaluation of hydrocarbon polluted soil in Obitti oil field in Ohaji Egbema was investigated to ascertain the presence of bacterial community in crude oil-polluted soil. Ohaji Egbema has experienced crude oil pollution in recent times due to the presence of crude oil exploration facilities including the Obitti oil field. Soil samples were collected from four (4) different points within the oil field with varying degree of crude oil pollution along with their corresponding control samples (unpolluted soil). The total culturable heterotrophic bacteria (TCHB), total culturable hydrocarbon utilizing bacteria (TCHUB), pH and total petroleum hydrocarbon (TPH) concentrations were monitored using standard procedures. The total culturable heterotrophic bacterial count ranged from 0.45±2.0x105 Cfu/g to 2.12±2.6x105 Cfu/g while total culturable hydrocarbon utilizing bacterial count ranged from 0.56±2.1x104 Cfu/g to 1.35±2.6x103 Cfu/g. pH ranged from 0.11±4.0 to 1.10±5.0 for the polluted soil samples while a range of 0.10±5.7 to 1.00±7.2 was recorded for the control samples. The TPH analysis revealed a high concentration of 9.51±5747.13 mg/kg to 3.45±7214.82 mg/kg for polluted soil samples which is above the DPR intervention limit of 5000 mg/kg for soils while the control samples recorded a range of 5.41±3118.29 mg/kg to 8.21±4285.02 mg/kg. This study has therefore revealed the ability of indigenous bacterial population to strive despite crude oil pollution and as such these hydrocarbon impacted sites can be harnessed for the isolation of hydrocarbonoclastic bacteria which can serve as a bio-resource for the effective bioremediation of hydrocarbon impacted environment.
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Bacterial species identification

51%
Kshikhundo R., Itumhelo S.
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EN
The traditional methods of bacterial identification are based on observation of either the morphology of single cells or colony characteristics. However, the adoption of newer and automated methods offers advantage in terms of rapid and reliable identification of bacterial species. The review provides a comprehensive appreciation of new and improved technologies such fatty acid profiling, sequence analysis of the 16S rRNA gene, matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF), metabolic finger profiling using BIOLOG, ribotyping, together with the computational tools employed for querying the databases that are associated with these identification tools and high throughput genomic sequencing in bacterial identification. It is evident that with the increase in the adoption of new technologies, bacterial identification is becoming easier.
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