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  1. 1 Journal of Applied Genetics

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  1. 1 1996

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  1. 1 Kaminski S.

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Dde I RFLP at the 5'region of bovine kappa-casein gene

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Kaminski S.
Journal of Applied Genetics
|
1996
|
vol. 37
|
issue 2
173-178
EN
The bovine kappa-casein (CASK) gene is known as a potential quantitative trait locus in dairy cattle breeding.However, the molecular basis of the effect of the CASK allele B on different milk properties remains unclear.In this report, a 214 bp fragment of the 5' untranslated region of CASK gene containing 5 potential consensus sequences for different transcription factors was PCR-amplified to find RFLPs.A Dde I RFLP was identified.IN population of 112 Bos taurus (86 cows and 26 bulls of Polish Black and White crossbred Holstein-Friesian) and 7 Bisons bonasus individuals, 7 had no recognition sites for Dd I, 23 were hetrozygous and 89 were cut completly into two fragments.
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