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EN
after solvent refining process were used as a carbon source for cultivation of and ATCC 20509. Biomass yield varied from 3.8 g d.m./l to 19.9 g d.m./l and lipids yield varied 1.0 g/l to 10.6 g/l. Lipids utilization in medium varied from 15.0% to 85.4%. Process efficiency, and percentage composition of fatty acids of intracellular lipids and grease remaining in the medium depend first of all on medium composition, type of microorganisms used, temperature and time cultivation.
EN
The production of Single Cell Protein in the batch culture from crude rapeseed oil and waste fatty acids using Y. lipolytica strains was studied. The effect of nitrogen sources, lipid concentration and pH value on the yeast growth and biomass yield was also investigated. Yarrowia lipolytica ATCC 8661 was the best strain for SCP production on both lipid substrates. The specific growth rate and biomass yield were m = 0,19 - 0,35 h-1 and Yx/s = 0,68 - 1,07 respectively, depending on the culture condition. With the above yeast strain it was possible to carry out the fermentation at low pH 3,5 without any significant lowering of kinetic and yield parameters.
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