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Heteroplasmy in plants

100%

Woloszynska M., Oziemlak A., Janska H.

Biotechnologia

2001

issue 1

42-46

Heteroplasmy is the state characterised by the presence of more than one type of mitochondrial or chloroplast genome in one organism. Infrequent recombinations across short repeated sequences often lead to heteroplasmy in higher plant mitochondria. Different Phaseolus species have been examined in order to understand the dynamics of heteroplasmy originating through recombination mediated by the 315 bp repeated sequence. Two techniques were applied to detect heteroplasmy: Souhern hybridization with prolonged autoradiography and PCR amplification followed by hybridization or reamplification. In all examined genomes the four recombination forms were detected. However, these forms do not occur in the same relative amounts. Moreover, in the examined genomes different recombination forms exist at the predominant/substoichiomeric level, but always only two of them are predominant. Based on these results and the previous data, we suggest that the changes in the heteroplasmic population of mitochondrial molecules in plants may offer the source of genetic variation in the course of evolution.

Phaseolin seed variability in common bean (Phaseolus vulgaris L.) by capillary gel electrophoresis

75%

Salmanowicz B. P.

Journal of Applied Genetics

2001

vol. 42

issue 3

269-281

Phaseolin, the major seed storage protein of Phaseolus vulgaris from forty-four wild and cultivated accessions, was studied using sodium dodecyl sulphate-capillary gel electrophoresis (SDS-CGE). In total, eleven phaseolin profiles, revealing polypeptide subunit variation in the range from 45.6 kDa to 54.4 kDa, were recorded. The number of polypeptide subunits recorded in particular profiles varied from 3 to 6; in total, eight phaseolin subunits were distinguished in the examined material. Ferguson plot analysis was used to correct non-ideal behaviour of phaseolin polypeptide subunits in capillary gel electrophoresis in the presence of SDS. The obtained results are compared to electrophoretic data received by slab polyacrylamide gel electrophoresis. The SDS-CGE method appears to provide a powerful tool for disclosure of phaseolin subunit variability.

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1 Biotechnologia

1 Journal of Applied Genetics

2 2001

1 Janska H.

1 Oziemlak A.

1 Salmanowicz B. P.

1 Woloszynska M.

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Heteroplasmy in plants

Phaseolin seed variability in common bean (Phaseolus vulgaris L.) by capillary gel electrophoresis