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B-cell chronic lymphocytic leukemia-associated nuclear antigens

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Rogalińska M., Błoński J. Z., Robak T., Kiliańska Z. M., Małgorzata Rogalińska - University of Łódź D. o. C., Jerzy Z. Błoński - Medical University of Łódź D. o. H., Tadeusz Robak - Medical University of Łódź D. o. H., Zofia M. Kiliańska - University of Łódź D. o. C.
Acta Universitatis Lodziensis. Folia Biologica et Oecologica
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2008
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vol. 4
EN
One- and two-dimensional polyacrylamide gel electrophoresis were used to compare the composition of nuclear polypeptides from normal and В-cell chronic lymphocytic leukemia mononuclear cells. Against two electrophoretically-specific nuclear proteins with molecular weight of 38/39 and 44/46 kD a from leukemic cells rabbit sera were obtained. As it was analyzed by Western blot technique the available antisera recognized the 38/39 kDa antigen in 53 of the 56 (94.6%), while the 44/46 kDa in 46 of the 49 (93.9%) of examined В-CLL nuclear fraction preparations, but not in normal ones. The pi values of described leukaemia-specific antigens were determined; p38/39 had pi in the range of pH 6.55 -7.00 and p44/46 - in the range of pH 6.2-6.4.
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