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DNA molecules site-specific immobilization and their applications

100%
Zhang X., Hu H.
Open Chemistry
|
2014
|
vol. 12
|
issue 10
977-993
EN
In addition to its role as a carrier of genetic information, DNA has been recognized as a construction material for the assembly of different objects and structural arrangements with nanoscale features. As a result of DNA’s self-recognition properties (based on the specific base-pairing of G-C and T-A), monolayer films of nucleic acids on solid supports have attracted an escalating attentions. Recently, numerous novel materials based on two-dimensional (2D) and three-dimensional (3D) DNA structures have been reported, which extends their utility to a large number of appliations. This review paper intends to be a new and comprehensive overview of recent strategies to site-specifically immobilized DNA on various materials, including carbonaceous substances, gold, and silica substrate, emphasizing the applications of site-specific DNA nanostructure-based devices for diagnostic, bioanalytical, food safety and environmental monitoring. Additionally, an up-to-date perspective is proposed at the end of this review.
2
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Recent progress in the application of glow-discharge electrolysis plasma

100%
Ren J., Yao M., Yang W., Li Y., Gao J.
Open Chemistry
|
2014
|
vol. 12
|
issue 12
1213-1221
EN
Non-equilibrium plasma makes it is possible to modify surface chemistry, synthetize polymer materials, and oxidize some organic compounds completely by generation of energetic and chemically active species in gas or liquid phases. Glow-discharge electrolysis plasma (GDEP) has been intensely studied for applications in chemistry and in material, environmental, and biomedical engineering during the last few years because of the very highly active chemical species produced during the glow-discharge electrolysis (GDE) process. A brief review is already available regarding applications of glow-discharge electrolysis plasma technique in chemistry and environmental science during the past decade. For convenience of discussion, some papers from prior years are also cited. The contents of this review are focused on the degradation of persistent pollutants, surface modification of materials, and preparation of functional polymers.
3
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Developments of instruments and methods related with high-speed countercurrent chromatography and their applications in research of natural medicines

88%
Yao S., Cao Y., Jia C.-m., Wang Y., Song H.
Open Chemistry
|
2012
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vol. 10
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issue 3
417-432
EN
High-speed countercurrent chromatography (HSCCC) is a liquid-liquid separation chromatographic technique which uses no solid supporting medium. During its rapid development in the last 30 years, great progress has been made in the instrumentation, the basic study and application of HSCCC. It has significant advantages over other instrumental separation techniques, in its high efficiency and continuous processing capability. In recent years, HSCCC has been widely used in research and development of natural medicines and functional foods, including preparative separation, fingerprint analysis and screening of bioactive constituents. A review of the technique is presented in this paper. [...]
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