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1

Distribution of indole-3-acetic acid in seawater and marine sediments of the Gulf of Gdansk

100%
Mazur H.
Oceanological Studies
|
1994
|
issue 67/10
27-32
EN
HPLC method was used to detect the presence of auxin indole-3-acetic acid (IAA) in seawater and sediments of the Gulf of Gdansk.In spring considerably higher concentration of IAA was measured in sediments and near-bottom water than in surface water.The concentration of IAA changes with season and sampling statin and it seems to depend on process undergoing in the marine environment.
2

Micropropagation of Salvia officinalis L. by shoot tips

100%
Grzegorczyk I., Wysokinska H.
Biotechnologia
|
2004
|
issue 2
212-218
EN
In this study, optimum conditions for the micropropagation of Salvia officinalis by shoot tips were determined. Shoot tips from five-week-old shoots grown in in vitro culture were used as explants. The explants were incubated on MS agar medium supplemented with 0.57 ?mol/l IAA and various concentrations of cytokinins (BAP, zeatin, TDZ or kinetin). The best results were obtained when BAP at the concentration of 2 ?mol/l was used as cytokinin. Under these conditions after 5 weeks more than 90% shoot tips formed axillary buds or shoots and almost 3 shoots per one explant were obtained. An average length of shoots was 2.5 cm. For root induction the most suitable was ? MS agar medium without growth regulators.
3

The influence of exogenous growth regulators on germination and conversion in somatic embryos of Norway spruce (Picea abies (L.) Karst.)

100%
Latkowska M. J., Wojciechowska K.
|
EN
Modification of the medium composition was a trial to improve germination and conversion in somatic embryos of Norway spruce. The mature embryos germinated for 1 week in the medium supplemented with either auxin (IAA, IBA, NAA ? 0.05; 0.25; 0.1 or 0.5 muM) or gibberellin (GA3, GA4+7 - 25; 50; 125 or 250 mg dm-3). The hormone-free medium used for the next 2 weeks of germination was enriched or not with activated charcoal (10 mg dm-3). The auxin applied in the medium did not affect embryo germination nor root and shoot growth. GA3 stimulated root and shoot growth in germinating somatic embryos, whereas the highest concentration of GA4+7 completely inhibited embryo germination.
4

The effects of 2,4-D, NAA and picloram on callus induction and somatic embryogenesis in ornamental asparagus

88%
Kepczynski J., Zielinska S., Kepczynska E.
Biotechnologia
|
2001
|
issue 2
170-173
EN
In this study we examined the effect of 2,4-D, NAA and picloram at 1.5, 3.0 and 4.5 mg/l in MS medium on callus induction and somatic embryogenesis in Asparagus densiflorus cv. Sprengeri and Asparagus plumosus. The callus formation of both shoot tips and nodal explants from A. densiflorus after 8 weeks of culture on MS medium supplemented with NAA and picloram (1.5, 3.0 mg/l) was observed. However, callus on medium containing 2,4-D was occasionally induced. On medium supplemented with picloram shoot tip explants of Asparagus plumosus showed callus inducing capacity. After four weeks of callus culture on hormone-free medium the globular embryos were achieved. On callus induced NAA organogenesis was observed.
5

The effect of transformation with iaglu gene on growth and development of trangenic strawberry in in vitro cultures

75%
Wawrzynczak D., Sowik I., Michalczuk L.
Biotechnologia
|
2004
|
issue 2
46-53
EN
The aim of the presented work was to study the effects of changes of endogenous indole-3-acetic acid (IAA) metabolizm on in vitro shoot proliferation and rhizogenesis of transgenic strawberry shoots carrying maize IAA-glucose synthase gene (iaglu). Four iaglu-transformed strawberry clones and nontransformed 'Kaster' shoots served as a plant material for the study. The analysis of free and conjugated IAA level in leaves of transgenic and control strawberry plants showed that iaglu-containing strawberry clones had significantly higher level of ester conjugated IAA, but the level of free hormone was only slightly decreased or comparable to the control plants. iaglu-transformed clones had significantly higher proliferation rate and formed more roots than the control shoots. One of the iaglu-transformed clones had significantly shorter and other two ? longer roots than the control plantlets.
6

Densytometric analysis of diosgenin in extracts from callus tissue of Polygonatum verticillatum (L.) All.

75%
Szybka-Hrynkiewicz P., Janeczko Z.
Biotechnologia
|
2004
|
issue 2
93-99
EN
In this study, we examined the effect of plant growth regulators (2, 4-D, 6-BAP) and steroidal hormone (17-beta-estradiol) on a quantity of diosgenin in callus tissue of Polygonatum verticillatum (L.) All. The callus grown in in vitro conditions on MS medium with: 1/1, 2/2, 5/5, 10/10 ([mg/dm3] 2,4-D/[mg/dm3] 6-BAP) and 1/1/1, 2/2/2, 5/5/5, 10/10/10 ([mg/dm3] 2,4-D/[M/dm3] 17beta-estradiol/[mg/dm3] 6-BAP) was used for the densytometric analysis to determine the presence of diosgenin. The content of diosgenin ranged from 0,0642 to 1,1458%. An increase in diosgenin content due to addition of 6-BAP and 2,4-D was observed. The developed procedure was estimated by statistical analysis which indicates that it can be used for routine analysis.
7

Germin genes expression induced by stress factors in transgenic tobacco plants (Nicotiana tabacum var. Xanthi)

63%
Nowakowska J.
Biotechnologia
|
2001
|
issue 2
152-164
EN
Wheat germin, a glycoprotein mainly localized in the cell wall of monocots, dicots and pine (Pinus caribaea Morelet), is a specific marker of the onset of growth in germinating seeds. There are two genes gf-2.8 and gf-3.8 of germin, isolated from wheat (Triticum aestivum). Because of their structure and biochemistry, both of the germin's isoforms have been now assimilated to the oxalate-oxidase enzyme (E.C. 1.2.3.4). Several constructs of the gf-2.8 and gf-3.8 genes promoters deletion were fused with uidA gene and tested for the GUS activity in the transgenic tobacco plants. The aim of this work was to evaluate whether some environmental factors induce the activity of germin. Hormonal treatment (auxins 2,4-D, NAA, gibberellin GA3), salt treatment, salicylic acid, heavy metals (Mn, Fe, Co, Ni, Cu, Zn, Cd, Hg, As) and A1 induced high GUS activity in root apex, cotyledons and hypocotyls in transgenic seedlings and in leaves of the tobacco plants transformed with the entire and one partially deleted construct of the gf-2.8 gene. Neither cold nor heat shock, enhanced significant GUS activity. The gf-3.8 - GUS constructs of germin did not give a positive response to any factors mentioned above. Thus, only the gf-2.8 protein seems to be regulated by some factors. The gf-2.8 oxalate-oxidase activity could be then involved in general stress-induced signalling in higher plants
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