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1

Secondary metabolites from hairy roots of Tanacetum parthenium

100%
Stojakowska A., Kisiel W.
Biotechnologia
|
2003
|
issue 3
87-94
EN
Feverfew (Tanacetum parthenium (L.) Sch. Bip.) is an ornamental and medicinal plant. Its preparations are mainly used for migraine prophylaxis. Complex mixture of T. parthenium secondary metabolites contain among others, sesquiterpene lactones and spiroketalenolether diacetylenes. From the roots of plant transformed with Agrobacterium rhizogenes LBA 9402 four diacetylenes and two coumarins were isolated, including the sesquiterpene coumarin ether 9-epipectachol B, a new natural product. The influence of exogenously applied salicylate and methyl jasmonate on diacetylene accumulation pattern was studied. Both signal molecules induced significant changes in the diacetylene accumulation suggesting that the compounds are involved in the plant defence.
2

Stimulation of anthocyanin production by two-phase growth system, in callus of Rudbeckia hirta

75%
Luczkiewicz M., Cisowski W.
Biotechnologia
|
1999
|
issue 4
199-210
EN
Biotechnological research to achieve and then increase anthocyanin production in callus tissue of R. hirta L., involved testing a number of growth media, modified with growth regulators. The evaluation of the growth of propagated tissues, their water contents and in particular their ability to produce anthocyanins, led to the development of an original two-phase growth system. This new system has the advantages of both growth-stimulating media and pigment production media. As a result of the research, callus tissue was obtained on a two-phase growth medium, which was made of modified Schenk-Hildebrandt medium (growth phase) and Miller's medium (production phase). The callus synthesised a 12-component anthocyanin compound complex, which constituted 2,18% of dry mass. This is a considerable amount compared to 0,28% in the natural plant. Phytochemical analysis (TLC, HPLC, PC, UV, 1H-NMR) of the anthocyanin complex isolated from callus produced with the two-phase system proved that the dominating compounds in the pigment complex were: cyanidin-3-0-(6-0-malonyl-b -D-glucopyranoside) and cyanidin-3-0-(b-D-glucopyranoside).
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