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Micropropagation of Arnica montana L. and sesquiterpene lactones in regenerated plants

100%

Weremczuk-Jezyna I., Wysokinska H.

Biotechnologia

2000

issue 4

118-122

Plants of Arnica montana L. were micropropagated from shoot tips. Shoot proliferation was obtained on Murashige and Skoog (MS) medium containing auxin (IAA 0.5 M/l) and cytokinin (BAP, Z, TDZ). BAP (0.25 M/l) and Z (0.5 M/l) were most effective for proliferation and elongation of shoots. Under these conditions, about 6 shoots per explant were obtained. Shoot proliferation was also achieved in the presence of TDZ (0.05 M/l). However, when used in higher concentration (0.2-10 M/l), thidiazuron caused shoot vitrification. Rooting of shoots was induced an MS medium without growth regulators. The plantlets were transplanted into pots. Phytochemical analysis showed that 4-week-old plantlets produced sesquiterpene lactone.

Transformed root cultures of Arnica montana L. ? of secondary metabolites isolation

88%

Weremczuk-Jezyna I., Wysokinska H.

Biotechnologia

2004

issue 2

54-61

The hairy roots of A. montana were achieved by the infection of sterile leaves with Agrobacterium rhizogenes (strain LBA 9402). The transformation was confirmed by opin and PCR analysis. In the investigation, the optimum contitions of Hairy roots growth were characterized. The best results were achieved in liquid Gamborg medium which included half-strenght macro- and microelements (1/2B5) and 50 g/l sucrose. Phytochemical analysis showed that hairy roots of A. montana produced arnifolin and chlorogenic acid.

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2 Biotechnologia

1 2004

1 2000

2 Weremczuk-Jezyna I.

2 Wysokinska H.

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Micropropagation of Arnica montana L. and sesquiterpene lactones in regenerated plants

Transformed root cultures of Arnica montana L. ? of secondary metabolites isolation