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1

Estimation of callus formation capability of anthers obtained as a result of crossing Linola cultivar with other linseed cultivar plants

100%
Wielgus K., Mankowska G., Bialas W.
Biotechnologia
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2008
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issue 2
84-89
EN
Identification of responsive genotypes and development of an efficient and cost-effective doubled haploid production system in linseed (Linum usitatissimum L.) is required for application in practical breeding program. Although the technique has been improved in recent years, there is limited data on the inheritance of callogenesis and plant regeneration in linseed anther culture. The objective of this research was to estimate the influence of the ?Linola' cultivar genome and other cultivars used for crossing with 'Linola' plants in a process declining during the anther cultures in F1 generation. 3144 anthers altogether were placed on the medium, on average 393 from each population. Hybrid lines obtained as a result of crossing 'Linola' x 'A.C. Emmerson' were not capable to form callus. The anthers from crossing of 'Linola' x 'Szafir', 'Linola' x 'Opal' and 'Linola' x 'Bionda' were characterized with higher than average capacity to form callus, while the anthers from crossing of 'Linola' x 'Norlin', 'Linola' x 'Vinny' and 'Linola' x 'Oliwin' had lower potential of forming callus.
2

Production and evaluation of doubled haploid wheat lines

100%
Pauk J., Kertesz Z.
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vol. 38
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issue 4
425-435
EN
F1, F2 populations as well as F3 and F4 wheat lines coming from more than 300 cross combinations have been used for androgenic wheat studies and more than 5000 haploid plants were produced. Promising doubled haploid lines were tested together with lines produced in the conventional way in yield trials. The two best doubled haploid lines overyielded the standard. On the basis of results related to the general combining ability it is advisible to cross parents with a good combining ability for characters such as high percentage of responding anthers and embryo/callus induction with parents indicating a good combining ability for green plant production. In this way ideal lines can be obtained for doubled haploid production.
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